【摘要】 [目的]探讨二甲基乙二酰基甘氨酸（dimethyloxaloylglycine,DMOG）对间充质干细胞（mesenchymal stem cells,MSCs）生物学特性的影响。[方法]以DMOG作用于P4代MSCs,分为DMOG 0、20、40和80μmol·L^-1组,以CCK-8法、流式细胞术、Transwell迁移法和Real-time q PCR依次检测不同浓度的DMOG对MSCs增殖能力、细胞周期、迁移能力以及成骨、成脂、成软骨和成神经分化能力的影响。[结果]DMOG各浓度组细胞生长曲线均接近S型,与DMOG 0μmol·L^-1组比较,20μmol·L^-1对MSCs增殖能力具有促进作用,40μmol·L^-1促进作用最明显,差异具有统计学意义（P<0.05）。与DMOG0μmol·L^-1组比较,DMOG处理可提高处于S期+G2/M期的MSCs细胞比例,差异具有统计学意义（P<0.05）。与DMOG 0μmol·L^-1组比较,DMOG20μmol·L^-1时促迁移作用显著（P<0.05）。DMOG处理后,MSCs仍保留了原有的成骨、成脂、成软骨、成神经分化能力,DMOG 20μmol·L^-1可显著促进MSCs成骨和成软骨分化能力,抑制MSCs成脂分化能力,差异具有统计学意义（P<0.05）,对MSCs成神经分化能力无显著影响（P>0.05）。[结论]不同浓度DMOG处理后,MSCs仍保留了原有的多向分化能力,其中20μmol·L^-1DMOG可促进MSCs成骨和成软骨分化能力,抑制其成脂分化能力,对其成神经分化能力无显著影响,同时可显著提高MSCs增殖、迁移能力。更多还原

【Abstract】 [Objective] To investigate the effect of dimethyloxaloylglycine（DMOG） on mesenchymal stem cells（MSCs） biological behavior in vitro. [Methods]The P4 generation of MSCs were treated with DMOG, and were divided into DMOG 0μmol·L^-1 group, DMOG 20μmol·L^-1 group, DMOG 40μmol·L^-1 group and DMOG 80μmol·L^-1 group. The CCK-8 assay, flow cytometry, transwell method, Real-time q PCR were adopted to detect the effects of DMOG on the proliferation, cell cycle, migration, and differentiation of MSCs. [Results]The cell growth curve of every group was close to S type after DMOG treatment（P<0.05）, compared with DMOG 0μmol·L^-1, the 20μmol·L^-1 processing began to promoted cell proliferation ability of MSCs（ P<0.05）,and the promotion effect of DMOG 40μmol·L^-1 was the most obvious（P <0.05）; cells proportion of S phase +G2/M phase in MSCs was improved with DMOG treatment（P<0.05）; compared with DMOG 0μmol·L^-1, the promotion effect of migration ability was statistically significant of DMOG 20 μmol·L^-1 processing（P<0.05）; MSCs still kept the original osteogenic, adipogenic, chondrogenic, neural differentiation abilities after treated with DMOG, 20 μmol·L^-1 treatment could obviously promote osteogenic and chondrogenic differentiation abilities（ P<0.05）, suppress adipogenic differentiation ability（P<0.05）.[Conclusion]After processing with different concentration of DMOG, MSCs still kept the original multipotent differentiation abilities. 20 μmol·L^-1 DMOG treatment could obviously promote osteogenic and chondrogenic differentiation abilities, suppress adipogenic differentiation ability, and could improve the proliferation and migration abilities of MSCs.更多还原