【摘要】 以48个鲜食葡萄品种为试材,通过室内离体叶盘接种霜霉病菌试验,对各品种的霜霉病抗性分级,结合对抗病相关基因PR1、PR5和NPR1的半定量RT-PCR试验,分析不同抗性级别品种在霜霉病菌侵染后表达模式的差异,以期为抗葡萄霜霉病的新品种选育提供参考依据。结果表明:48个品种中有30个品种对霜霉病高度敏感,7个品种对霜霉病敏感,4个品种中抗,6个品种高抗霜霉病,"金星无核"对霜霉病完全免疫。PR1和PR5基因在接菌前有本底表达,抗病和高抗品种接菌后均上调表达,感病品种接菌后无明显变化,仅"无核翠宝"和"红地球"有微量下调。NPR1基因接菌后在抗病品种中表达量上升,而在感病品种和高抗品种中均表现为半数品种上升,半数品种下降。更多还原

【Abstract】 In order to provide reference for the breeding of downy mildew resistance varieties,the resistance to downy mildew of 48 table grape varieties were tested by leaf disc inoculation indoor.Meanwhile,the expression patterns of resistance-related genes PR1,PR5 and NPR1 to downy mildew were investigated through semi-quantitative RT-PCR.The results showed that 30 varieties were highly susceptible,7 susceptible,4 resistant,6 highly resistant,‘Venus Seedless’ immune to downy mildew.The difference of PR1 and PR5 expression were not significantly in susceptible varieties except ‘Wuhecuibao’ and ‘Red Globe’,which were reduced slightly after inoculation.The expression of PR1 and PR5 were higher in the resistant ones after downy mildew infection.In susceptible varieties and high-resistant varieties,the expression of gene NPR1 was up-regulated in 50% varieties and down-regulated in the other ones,while the expression was up-regulated in all the resistant varieties.更多还原