【摘要】 [目的]建立快速测定D-虫荧光素的液相色谱-质谱(HPLC-MS/MS)分析方法,并对荧光素的碎裂机理进行解析。[方法]采用Shim-pack VP-ODS(150 L×2.0 mm,5μm)色谱柱,甲醇、水梯度洗脱12min进行色谱分离;在质谱正离子模式下,用6对反应监测离子对(MRM)进行定性、定量分析。[结果]目标化合物在0.625～125.000μg/L线性关系良好(r=0.996 9),检出限为0.250μg/L(S/N为5)。[结论]该方法灵敏度高、选择性好,不仅可用于萤火虫发光机理、发光强弱差异、发光颜色差异方面的研究,还可以用于其他需要定量测定荧光素的研究。更多还原

【Abstract】 [Objective]A rapid high performance liquid chromatography-mass spectrometry(HPLC-MS/MS)method was developed for the determination of D-Luciferin.The D-Luciferin fragmentation mechanism was analyzed.[Method]The HPLC separation was carried on the shim-pack VP-ODS column(150 L×2.0 mm,5 μm) with methanol and water eluting gradiently in 12 min.The D-Luciferin was detected by using 6 multiple reaction monitoring(MRM) in ESI positive mode of mass spectrometer. [Result]There was a good linear relationship within the range of 0.625-125.000 μg/L(r=0.996 9).The quantitative determination limit was 0.250 μg/L(S/N=5).[Conclusion]The method is sensitive and selective. It can be used to detect D-Luciferin rapidly in the firefly or other research.更多还原