Construction of Suppression Subtractive Hybridization Library for Testis of Male Tilapia Under the Stress of Methomyl

【摘要】 [Objectives] This study was conducted to construct forward and reserve libraries of suppression subtractive hybridization（SSH） in the testis of male tilapia under the stress of methomyl by using SSH technology. [Methods] Using male tilapia as the test animal, the forward and reserve libraries of SSH in the testis of tilapia under the stress of methomyl were constructed by using the SSH technology. [Results] 45 expressed sequence tags（ESTs） were obtained, and 25 expressed sequence tags were successfully noted, including 13 forward libraries and 12 reverse libraries. The genes with confirmed functions were classified into five types. Genes related to catalytic activity and cell characteristics were up-regulated, while genes related to structural molecule’s activity and biological process were down-regulated. The expression amount of integrin β1 was up-regulated, while serine/threonine protein kinase pim-3, Ca²⁺-ATPase, Na⁺-K⁺-ATPase and ribosomal protein L22 were down-regulated. [Conclusions] The research results could lay a foundation for revealing the molecular mechanism of methomyl’s reproductive toxicity to tilapia.更多还原

【Abstract】 [Objectives] This study was conducted to construct forward and reserve libraries of suppression subtractive hybridization（SSH） in the testis of male tilapia under the stress of methomyl by using SSH technology. [Methods] Using male tilapia as the test animal, the forward and reserve libraries of SSH in the testis of tilapia under the stress of methomyl were constructed by using the SSH technology. [Results] 45 expressed sequence tags（ESTs） were obtained, and 25 expressed sequence tags were successfully noted, including 13 forward libraries and 12 reverse libraries. The genes with confirmed functions were classified into five types. Genes related to catalytic activity and cell characteristics were up-regulated, while genes related to structural molecule’s activity and biological process were down-regulated. The expression amount of integrin β1 was up-regulated, while serine/threonine protein kinase pim-3, Ca²⁺-ATPase, Na⁺-K⁺-ATPase and ribosomal protein L22 were down-regulated. [Conclusions] The research results could lay a foundation for revealing the molecular mechanism of methomyl’s reproductive toxicity to tilapia.更多还原