【作者】 胡俐；

【导师】 王德辉； 余洪猛； 顾瑜蓉；

【作者基本信息】 复旦大学 ， 耳鼻咽喉科学， 2010， 博士

【摘要】 目的研究T细胞亚型相关的五种细胞因子IFN-γ、IL-4、IL-17、IL-10、TGF-β在鼻息肉中的表达情况,探讨各种细胞因子在过敏与非过敏性鼻息肉形成过程中所起作用。方法研究对象包括非过敏组10例,过敏组15例,正常对照组10例。采用免疫组织化学荧光检测法研究五种细胞因子在过敏与非过敏性鼻息肉形态学表达上的差异,Western Blot方法定量测定细胞因子IFN-γ、IL-4、IL-17、IL-10、TGF-β的蛋白表达水平。结果免疫荧光检测结果显示IFN-γ、IL-4、IL-10、TGF-β在非过敏组与过敏组鼻息肉粘膜下均有较明显的表达,而IL-17在过敏组表达较非过敏组明显。Western Blot结果显示过敏组、非过敏组IFN-γ、IL-4、TGF-β蛋白表达水平均较正常组升高(P<0.05),但两组间蛋白表达量并无明显统计学意义(P>0.05)。而正常组、过敏组与非过敏组三组间IL-17和IL-10蛋白表达水平均无明显统计学意义(P>0.05)。结论过敏性、非过敏性鼻息肉中细胞因子IFN-γ、IL-4、TGF-β表达均增多,可能在鼻息肉的炎症反应过程中起着重要的作用。IL-17和IL-10蛋白变化不明显。T细胞亚型相关细胞因子在过敏与非过敏性鼻息肉中的蛋白表达无明显差异性。目的研究T细胞亚型四种特异性转录因子T-bet、GATA-3、RORγt、Foxp3基因的定量表达,探讨四种基因在过敏与非过敏性鼻息肉的形成过程中所起的作用。方法研究对象包括非过敏组10例,过敏组20例,正常对照组10例。采用定量PCR分析四种基因的转录情况,比较其在过敏与非过敏性鼻息肉中表达的差异性。结果非过敏组T-bet基因表达水平较正常组、过敏组均有升高,差别具有统计学意义(P<0.05),但过敏组与正常组相比无明显统计学意义(P>0.05)。非过敏组GATA-3基因表达水平较正常组升高,差别具有统计学意义(P<0.05),而过敏组与正常组、非过敏组之间均无明显统计学意义(P>0.05)。正常组、非过敏组与过敏组三组之间RORγt、Foxp3基因表达水平无明显统计学意义(P>0.05),但非过敏组RORγt基因表达水平有明显升高趋势,过敏组与非过敏组Foxp3基因表达水平较正常组有明显降低趋势。结论非过敏性鼻息肉T-bet、GATA-3基因表达水平升高,过敏与非过敏性鼻息肉Foxp3基因表达水平均有降低趋势。T细胞亚型特异性因子除T-bet基因外GATA-3、RORγt、Foxp3在过敏与非过敏性鼻息肉中的表达均无明显差异性。Th1、Th2、Th17细胞可能在非过敏性鼻息肉的发病过程中起着更为重要的作用,Treg细胞可能对过敏与非过敏性鼻息肉均有抑制作用。更多还原

【Abstract】 Objectives:To quantificate the expression of T-cell related cytokines in nasal polyps and explore their roles in the development of allergic and non-allergic nasal polyps.Methods:Ten allergic and fifteen non-allergic patients with nasal polyps were studied. The T-cell subtype cytokines (IL-4, interferon [IFN]γ, IL-10, IL-17, and transforming growth factor [TGF]β) were analyzed by immunofluorescence. The levels of cytokine proteins were measured by Western Blot.Results:IFN-γ, IL-4, IL-10 and TGF-βwere expressed apparently in submucosa of all patients, but IL-17 was not easily observed in non-allergic ones by immunofluorescence. Increased levels of IFN-γ, IL-4 and TGF-βwere found in both allergic and non-allergic nasal polyps compared with control tissues (P< 0.05) However, there was no significances between them (P>0.05). Furthermore, the levels of IL-17 and IL-10 have no differences among contol tissues, allergic and non-allergic nasal polyps (P>0.05)Conclusions:Increased levels of IFN-γ, IL-4 and TGF-βwere found in both allergic and non-allergic nasal polyps, which suggested IFN-γ, IL-4 and TGF-βmay play important roles in the inflammation of nasal polyps. There was no differences of T-cell cytokines between allergic and non-allergic nasal polyps. Objectives:To quantificate the gene expression of T-cell Subtype characterized transcription factors in nasal polyps and explore their roles in the development of allergic and non-allergic nasal polyps.Methods:Ten allergic and twenty non-allergic patients with nasal polyps were studied. Expression of T-bet, GATA-3, Foxp3, and RORγt mRNA was detected by real-time polymerase chain reaction.Results:Expression of T-bet and GATA-3 mRNA was upregulated in non-allergic nasal polyps (P< 0.05). There was no significant differences on levels of T-bet and GATA-3 mRNA between allergic nasal polyps and control tissues (P>0.05). The levels of RORγt、Foxp3 mRNA have no differences among contol tissues, allergic and non-allergic nasal polyps (P>0.05). But markedly upregulated tendency of ROR Y t mRNA was showed in non-allergic ones and downregulated tendency of Foxp3 mRNA was manifested in all patients. Only the level of T-bet mRNA of non-allergic polyps was higher than allergic nasal ones (P< 0.05)Conclusions:The findings demonstrated that Th1, Th2 and Th17 cell may play a more important role in non-allergic nasal polyps. Treg cell was likely to inhibit the development of both allergic and non-allergic nasal polyps.更多还原