节点文献
参芪扶正注射液对化疗增效作用的分子机制研究
【作者】 丁治国；
【导师】 李乃卿；
【作者基本信息】 北京中医药大学 ， 中西医结合临床， 2010， 博士
【摘要】 1目的探讨参芪扶正注射液对化疗干预下小鼠肝转移癌组织基因表达谱的影响2方法2.1构建结肠癌肝转移小鼠模型选用BALB／c小鼠,将传代三次后的小鼠结肠癌C26瘤细胞液脾内注射后切除脾脏,建立实验模型。2.2分组给药造模小鼠共20只,分为两组,每组10只。5-氟尿嘧啶组(5-Fu组)；5-氟尿嘧啶+参芪扶正注射液组(5-Fu+参芪组)。2.3观察指标接种瘤株后每日称重,观察其活动、饮食及肿瘤发展情况。造模后第12天处死荷瘤小鼠,将肝转移癌组织取出称重后迅速液氮冻存备用。进行样品核酸制备、基因芯片检测及差异基因筛选,将筛选后的差异基因数据录入博奥生物有限公司分子功能注释系统进行生物信息学分析,解释药物的作用机理。3结果3.1造模成功率100%；3.2基因表达分析参芪扶正注射液可显著下调基因282个；上调基因13个。4结论参芪扶正注射液可能通过显著影响恶性肿瘤的多条信号转导通路,包括氧化磷酸化、嘌呤及嘧啶代谢、血管内皮生长因子信号转导通路、黏着连接、黏着斑等,实现对化疗的增效作用。
【Abstract】 1 ObjectiveTo explore effects of ShenQiFuZheng injection on gene expression profile of liver metastasis cancer in mice suffering chemotherapy.2 Methods2.1 Constructing mouse model of liver metastasis cancerSelecting BALB/c mice, establish the experimental models by cutting spleens after injecting C26 mouse colon cancer cells in them.2.2 Group and Administration20 Mouse models of liver metastasis cancer were set up and then randomly divided into two groups:5-Fu group, Shenqi Fuzheng Injection+5-Fu group.2.3 ObservationMice were weighted every day after being inoculated tumor cells. Observe the activity, appetite and the development of tumors. Tumor-bearing mouse were sacrificed at the thirteenth day. Tumor tissues were weighed and reserved with liquid nitrogen, then prepare the nucleic acid, detect the gene chips and analyse the genes with bioinformatics.3 Results3.1 All mouse models were set up sucessfully;3.2 Gene expressionShenQiFuZheng injection could up-regulate 13 genes and down-regulate 282 genes.4 ConclusionsShenQiFuZheng Injection can affect Several signal pathways in the tumor, including oxidative phosphorylation, purine metabolism, pyrimidine metabolism, VEGF signaling pathway, adherens junction, focal adhesion.