【作者】 金渊真；

【导师】 李忠仁；

【作者基本信息】 南京中医药大学 ， 针灸推拿学， 2009， 博士

【摘要】 癫痫是一组以反复发生的大脑神经元异常放电所致的暂时性中枢神经系统功能失常为特征的慢性疾病。按照神经元的部位和放电范围,功能失常可能表现为运动、感觉、意识、行为等不同障碍,或兼有之。根据不同的临床表现,西医常分为大发作、小发作、局限性发作、精神运动性发作等。中医属于“痫证”,“癫疾”或“羊癫风”的范畴,并认为大多数由于七情失调,先天因素,脑部外伤,饮食不节,劳累过度,或患病后,造成脏腑失调,痰浊阻滞,气机逆乱,风阳内动所致,而“顽痰”是为最重要的原因之一。上述癫痫的病因病机,相互兼杂,互为因果,变化多端,在发病过程中往往多种因素互存的。其疾病特点属于本虚表实,以脏腑亏损为基础的病理变化。最近,一氧化氮在神经科学研究中的作用受到研究者的重视。一氧化氮(nitric oxide,NO)是兼有细胞间第二信使功能和细胞毒性作用的气体物质。许多资料证实癫痫发作时伴随着NO的大量产生,过量的NO便会导致细胞功能紊乱,具有并参与多种生理功能及病理生理过程。本文从理论和实验两部分进行了系统的研究,研究了古代文献中的有关癫痫论述和进行了相关的电针对该病脑电图及一氧化氮和一氧化氮合酶的实验研究。本实验研究中,笔者通过针灸疗法治疗青霉素诱导癫痫大鼠模型的大鼠行为观察、大脑皮质和肝脏组织的一氧化氮和一氧化氮合酶的变化及和大鼠脑电图非线性动力学分析。结果表明:致癫模型的脑和肝脏内NO,NOS和NO/NOS含量显著上升,电针组NO,NOS和NO/NOS含量明显低于癫痫模型组,但假针刺组NO,NOS和NO/NOS含量高于针刺组。表明对大鼠电针穴位可抑制癫痫发作,可能与针刺调节一氧化氮有关系。电针显示大脑和肝脏NO,NOS和NO/NOS含量降低,从而减弱癫痫发作。对癫痫模型脑电活动进行非线性动力学分析,还有助于了解癫痫发生和发展的相空间结构和过程,判断癫痫灶的方位和有效地预防癫痫发作。从癫痫脑电活动的动力学角度来看,癫痫发作前期是发作间期向发作期的转化,它是脑电活动从高复杂度向低复杂度转化的阶段,而非线性动力学指标较其它两期发生明显变化。其结果对研究大脑的非线性动力学行为和其它非线性信号都具有一定的科学性和参考价值。同时也进一步证实了电针对癫痫模型大鼠的癫痫发作有一定的预防和抑制作用。更多还原

【Abstract】 Epilepsy is a paroxysmal event due to abnormal excessive hyper synchronous discharges from an aggregate of central nervous system (CNS) neurons, located in the neuron region and abnormal spike region, has abnormal expression-movement, sense, consciousness and etc. The main characteristic that distinguishes the different categories of seizures is whether the seizure activity is primarily generalized seizures or parietal seizures. Imbalance of the seven emotions, congenital deficiency, trauma, improper diet, prolonged illness, Zangfu function deficiency, disorder of Qi, internal wind stirring, and phlegm is the root cause.Nitric Oxide (NO) is effect on generated by the second-order reactions toxicology gas. Recently, most neuroscience researchers are observation for nitric oxide and neurologic disorders. According to research papers epileptic seizure was increased to nitric oxide and nitric oxide synthase (NOS) and over measure NO and NOS has confusion to cell signaling, NO has multiplicity of physiologic and path physiologic effects.Our research aims effect of the level of Nitric oxide, Nitric oxide syntheses in Brain and liver tissues and analysis EEG data in epileptic rats by electro acupuncture therapy.Throughout this work, induced epileptic seizure rat brain and liver tissue’s content of NO and NOS was increased, after electro acupuncture therapy resemble that the normal state. In the EEG data analysis by using both linear and nonlinear analysis, our analysis indicates that change that both before and after electro acupuncture therapy.Our research results show that electro acupuncture treatment by epileptic seizures scientific mechanisms and support to clinical research.更多还原