【作者】 赵钢；

【导师】 李令根；

【作者基本信息】 黑龙江中医药大学 ， 中西医结合临床， 2009， 博士

【摘要】 目的：1．成功建立家兔股动脉硬化闭塞症模型。2．通过动物实验观察康脉软胶囊对家兔股动脉硬化闭塞症模型的血清C-反应蛋白、白介素-6、基质金属蛋白酶-9、血栓素B₂，提高6-酮-前列腺素_1α的影响，以探讨康脉软胶囊治疗动脉硬化闭塞症的作用机理。方法：采用“高脂饲料喂养”、“电烧损伤股动脉内膜”、“股动脉环缩”、“牛血清白蛋白静脉注射免疫损伤”复合手段制成“家兔股动脉硬化闭塞症”模型；造模成功的新西兰大白兔54只随机分入康脉软胶囊治疗组、通塞脉片治疗组和模型空白组（未治疗）三组，每组18只；给药：康脉软胶囊组：0．56粒／Kg，用生理盐水稀释成10ml／Kg，灌胃，每日一次；通塞脉片组：0．75粒／Kg，用生理盐水稀释成10ml／Kg，灌胃，每日一次。模型组：生理盐水，10ml／Kg，灌胃，每日一次。各组每天上午按照10ml／kg标准灌胃给药一次。造模后通过病理、超声、造影及TXB₂、6-K-PGF_1α各项指标的检测验证该动物模型的建立成功；应用ELISA法检测三组模型给药前后血清C-反应蛋白、白介素-6、基质金属蛋白酶-9的变化情况。结果：1、通过病理、超声、造影及TXB₂、6-K-PGF_1α各项指标的检测证实了该动物模型的建立成功。2、康脉软胶囊可降低股动脉硬化闭塞症家兔血清C-反应蛋白、白介素-6及基质金属蛋白酶-9，降低血栓素B₂，提高6-酮-前列腺素_1α，并且明显优于通塞脉片治疗组（P＜0．01）。结论：1、根据相关指标判断该模型建立成功，符合动脉硬化闭塞症模型标准，可以进行相关炎症标志物与动脉硬化闭塞症关系的判断。2、康脉软胶囊通过降低血清C-反应蛋白、白介素-6、基质金属蛋白酶-9，降低血栓素B₂，提高6-酮-前列腺素_1α，对动脉硬化闭塞症的发生发展有着很好的抑制作用，起着积极的治疗作用。更多还原

【Abstract】 Objective:1.Successfully established rabbit model of atherosclerosisobliterans shares.2.Through animal experiments to observe the Kangmai softCapsule of the rabbit model of atherosclerosis obliterans of serum C-reactiveprotein,IL--6,matrix metalloproteinases-9,thromboxane B₂,to increase 6-keto-prostaglandin_1α the impact of Kangmai soft Capsule to explore the treatment of softgelatin capsules arteriosclerosis obliterans mechanism.Methods:The“high-fat diet,”“electric burn injury in femoral artery intima,”“femoral artery ring reduction,”“bovine serum albumin intravenous immune injury”means composite made of“Rabbit Femoral Arteriosclerosis Obliterans”model;successful model 54 New Zealand white rabbits were randomly divided intoKangmai soft Capsule treatment group,the treatment Tongsaimai model group andblank group（no treatment） three groups of 18;administrat:Kangmai soft CapsuleGroup A:0.56 / Kg,diluted with saline 10ml/Kg,orally,once a day;Tongsaimaitablets group:0.75 / Kg,diluted with saline 10ml/Kg,orally,once a day.Modelgroup:normal saline,10ml/Kg,orally,once a day.Every morning in each group inaccordance with the standard oral administration 10ml/kg time.Model adopted bypathology,ultrasound,angiography and TXB₂,6-K-PGF_1αtesting to verify theindicators of the animal model of success;the application of ELISA detection modelof the three groups before and after administration of serum C-reactive protein,IL-6,matrix metalloproteinases-9 changes.Results:1,by pathology,ultrasound,angiography and TXB₂,6-K-PGF_1αdetection indicators confirmed the successful establishment of animal models.2,Kangmai soft Capsule can reduce the share of soft capsules arteriosclerosisobliterans rabbit serum C-reactive protein,IL-6 and matrix metalloproteinases-9and lower thromboxane B₂,to increase 6-keto-prostaglandin_1α,and significantlysuperior Tongsaimai tablets in the treatment group（P＜0.01）.Conclusion:1,in accordance with the relevant indicators to judge the successof the model,in line with the standard model of arterial occlusive disease,can berelated to inflammatory markers and the relationship between arteriosclerosis obliterans judgments.2,Kangmai soft Capsule by reducing the serum C-reactiveprotein,IL-6,matrix metalloproteinases-9 and lower thromboxane B₂,toincrease6-keto-prostaglandin _1α,arteriosclerosis obliterans of the occurrence anddevelopment of a very good inhibitory effect,plays an active role in treatment.更多还原