【作者】 马文光；

【导师】 马宝璋；

【作者基本信息】 黑龙江中医药大学 ， 中医妇科学， 2009， 博士

【摘要】 目的：本文在综述了慢性盆腔炎古今研究概况的基础上，研究炎克宁V号治疗慢性盆腔炎的作用机制。研究方法：采用慢性盆腔炎的常见致病菌——大肠杆菌、金黄色葡萄球菌、乙型溶血性链球菌成功的制作了慢性盆腔炎大鼠模型，将大鼠模型随机分为正常组、假手术组、模型组、炎克宁V号高、中、低剂量组和中药对照组。分别应用光镜和电镜方法对各组大鼠子宫组织进行形态学观察；应用免疫组织化学反应法检测各组大鼠子宫组织中NF-κB、Bax和ICAM-1的表达；应用酶联免疫吸附测定(ELISA)法检测各组大鼠血清中TNF-α和IL-10含量；黄嘌呤氧化酶法检测血清中SOD含量；应用逆转录聚合酶链式反应(RT-PCR)法检测各组大鼠子宫组织INF-γmRNA、NF-κBmRNA和ICAM-1 mRNA的表达。结果：炎克宁V号各组与模型组比较能明显减轻子宫粘膜充血、水肿、炎性渗出及粘连，促进组织修复。其中炎克宁V号中、低剂量组优于中药对照组(P＜0．05)；炎克宁V号中剂量组NF-κB、Bax和ICAM-1表达与模型组、高剂量组和中药对照组有显著性差异(P＜0．05)；炎克宁V号明显减弱了INF-γmRNA、NF-κBmRNA和ICAM-1 mRNA在子宫组织中的表达(P＜0．05)；炎克宁V号中、低剂量组血清中TNF-α和IL-10含量明显低于模型组、高剂量组和中药对照组(P＜0．05)；炎克宁V号中剂量组血清中SOD含量明显高于模型组、炎克宁V号高剂量组和中药对照组(P＜0．05)。结论：通过本实验说明炎克宁V号能明显减轻组织充血、水肿、炎性渗出及粘连，促进组织修复；炎克宁V号能明显降低NF-κB及其下游产物ICAM-1、TNF-α、L-10、INF-γ的水平；炎克宁V号能够促进子宫组织Bax表达，从而促进炎症细胞的凋亡；炎克宁V号能够通过增加SOD活性，加快清除氧自由基；炎克宁V号可能通过抑制NF-κB及炎性介质、促进炎细胞的凋亡及氧自由基的清除，有利于慢性盆腔炎症的消除。炎克宁V号方是治疗慢性盆腔炎的有效方剂。更多还原

【Abstract】 Objective:The researeh was studied in having summed up the chronic pelvicinflammatory disease(CPID)forth on the basis to study a general situationfrom ancient times to modern times,the purpose was to explore theYankeNing V curing CPID effect mechanism.Methods:Adopt chronic common CPID pathogenic bacteria-bacillus coli,staphylococcus aureus,hemolysis streptococcus B to have establish the CPIDrats model.Successfully.Female Wistar rats were randomly classified intothe following different groups:the normal group,the simulative modelgroup,the model group,the high dose of YankeNingVgroup,the mediumdose of YankeNing V group,the low dose of YankeNing V group and thecontrol group of Chinese medieine.Applied glossy microscope and electronmicroscope respectively to detect the ultrastruetural changes of uterus ofevery group rats.The expressiom ofBax,NF-KB and ICAM-1 was detectedwith the method of immunohistochemistry.The content of TNF-a and IL-10of serum was detected with the method of ELISA and the content of SOD inserum was detected with the method of xanthine oxidase.The expressiom ofINF-γmRNA,NF-κB mRNA和ICAM-1 mRNA in uterus was by RT-PCR.Results:Compared with the model group,the three YankeNing V groups canobviously relieve hyperemia,edema and adherence,and accelerate therecovery of the tissues.The effete of the low dose of YankeNing V group andthe medium dose of YankeNing V group were superior to the control group ofChinese medicine(p＜0.05);The expressiom of Bax,NF-κB and ICAM-lin themedium dose of the YankeNing V groupe had notable difference with themodel group,the high dose group of the YankeNing V and the control groupof Chinesemedicine(p＜0.05);The YankeNingVgroupe obviously decreased the expression of NF-KB and ICAM-1 mRNA(p＜0.05);The contents ofTNF-a,IL-10 of the low dose of YankeNing V group and the medium dose oftheYankeNing V in serum was obviously lower than the model group,thehigh dose group of YankeNing V group and the control group of Chinesemedieine group (P＜0.05);The contents of SOD of the medium dose oftheYankeNing V in serum was significantly better than the model group,thehigh dose group of YankeNing V group and the control group of Chinesemedieine group (P＜0.05).Conc IU sions:Through this experiment,we can have the explainions:the YankeNing Vrelieve hyperemia,edema and adherence,and accelerate recovery of thetissues;The YankeNing V can obviously decrease NF-κB and its down streamproducts(ICAM-l,TNF-a,L-10,INF-γ);The YankeNing V can promote theexpression of Bax,and promote apoptosis of inflammatory cell;TheYankeNing V increase the aetivity of SOD,and aceelerate clearningoXradical;The YankeNing V treats CPID by inhibiting NF-κB and the relevantinflammatory medium,promoting apoptosis inflammatory cell and cleaningoxygen radical.The research has improved that the defined formula-Yankening V was effective when treating the common gynecologicaldisease-chronic pelvic inflammataotry disease (CPID).The YankeNingVisthe effective prescription for curing CPID.更多还原