【作者】 俞珊；

【导师】 魏品康；

【作者基本信息】 第二军医大学 ， 中西医结合临床， 2009， 博士

【摘要】 目的:观察消痰通络凝胶对骨癌痛大鼠机械痛阈值、体重、血清骨代谢标志物及辣椒素受体1表达的影响,探讨其治疗骨癌痛的作用和可能机制。方法:建立骨癌痛大鼠模型,分别以空白凝胶和消痰通络凝胶干预,观察大鼠行为变化,运用免疫组化、ELISA、实时荧光定量PCR及Western blot等分子生物学方法,检测血清Ⅰ型胶原吡啶交联终肽(ICTP)和骨特异碱性磷酸酶(BAP)及组织辣椒素受体1的表达(TRPV1)。结果:消痰通络凝胶干预后大鼠的机械痛觉阈值较模型组明显升高(p<0.05),消痰通络凝胶干预后大鼠血清ICTP水平较模型组明显降低(p<0.05),大鼠骨及下丘脑TRPV1蛋白及mRNA表达较模型组不同程度下调(p<0.05)。结论:消痰通络凝胶可有效抑制骨癌痛,其治疗骨癌痛的机理可能与减少骨吸收、下调骨及下丘脑TRPV1的表达有关。更多还原

【Abstract】 Objective: To observe the analgesic effects and mechanisms of Xiaotan Tongluo Gel (XTTL Gel消痰通络凝胶)on cancer pain by evaluating the mechanical allodynia threhold , body weight, markers of bone metabolism in serum and TRPV1 expression in a rat model of bone cancer pain (BCP). Methods: A rat model of BCP was established, the rats were applied with placebo gel and XTTL gel, observing the behavior of the rats, detecting ICTP , BAP and TRPV1 expression of the rats by methods of molecular biology as immunohistochemistry, ELISA, real-time PCR and Western blot. Results: The mechanical allodynia threshold of rats applied with XTTL Gel was significantly higher than that of model group(p<0.05),the ICTP in serum of rats with XTTL Gel was much lower than that of model group(p<0.05),and TRPV1 mRNA and protein expression in the bone and hypothalamus of the rats given XTTL Gel were lower than that of model group(p<0.05). Conclusion: XTTL Gel may alleviate bone cancer pain by inhibition of bone resorption and down regulation TRPV1 expression in the bone and hypothalamus .更多还原