脂肪来源Flk-1~+间充质干细胞三系分化过程中的MicroRNA表达

【作者】 谢杰；

【导师】 赵春华；

【作者基本信息】 中国协和医科大学 ， 临床医学， 2008， 博士

【摘要】 成体间充质干细胞是存在于成体内的一群有自我增殖和多系分化能力的细胞群体。近年来多有关于成体间充质干细胞向中胚层各种细胞类型分化以及向其他胚层细胞类型横向分化的报导,但对分化具体调控机制的研究报导少见。本次实验以脂肪来源成体间充质干细胞(AD-MSC)向胶质细胞、内皮细胞和上皮细胞三种不同胚层来源细胞的分化为模型,在前期microRNA芯片结果的基础上检测了miR-X和miR-Y在三种分化途径过程中的表达差异,并以miR-X在MSC向胶质细胞分化为例通过microRNA靶基因的预测探讨了microRNA在分化过程中可能发挥的后转录水平的调控作用。我们的结果显示miR-X和miR-Y在AD-MSC三系分化过程中表现出了各系特异性的表达时相特征,提示它们可能通过在后转录水平抑制功能性靶基因的表达而对AD-MSC的三系分化起重要的调控作用。更多还原

【Abstract】 Adult mesenchymal stem cells are a group of cells in adult tissue with the capacity of self-renewal and multi-lineage differentiation. A lot of reports have been published in recent years regarding the differentiation potential of mesenchymal stem cell to mesodermal as well as ectodermal and endodermal cells. However, the detailed regulatory mechanism underlying these processes remains unclear. In this research, adult mesenchymal stem cells were isolated from human adipose tissue and induced to differentiate into glial cells, endothelial cells and epithelial cells. Specific microRNAs were selected based on previous microarray results and their expression profiles during above processes were dectected. To further explore the possible mechanism of miR-X’s function in glial cell differentiation, its molecular targets were predicted through bioinformatic approach. Our data showed that miR-X and miR-Y had distinct expression profile in different lineage differentiation process, indicating that they may have important regulatory functions through post-transcriptional repression of functional target molecules.更多还原