节点文献
VEGF-D,TEM-8,Podoplanin在结直肠癌中的表达及其与局部淋巴结转移的相关性
Expression and Correlation with Lymph Node Envolvement of VEGF-D, TEM-8, and Podoplanin in Colorectal Cancer
【作者】 许毓敏;
【作者基本信息】 中国协和医科大学 , 普外科, 2008, 博士
【摘要】 目的结、直肠癌发展过程中出现的淋巴结转移被认为与其预后关系密切。淋巴生成因子及淋巴管密度被认为与肿瘤的淋巴结转移有关。VEGF家族中,VEGF-C和VEGF-D具有促进淋巴管生成的作用,而VEGF-D被认为是VEGF家族中唯一的一个专一的淋巴生长促进因子。Podoplanin首先在肾足细胞上被发现,后来发现它主要存在于成人的肺脏,淋巴内皮表面亦有表达,而且Podoplanin只存在于淋巴内皮,血管内皮并无表达。另外,Podolplanin和VEGFR-3共存,这证明Podoplanin是淋巴管的标记物。TEM(tumor endothelial marker)是新发现的肿瘤特异性的内皮标记物,一般认为和肿瘤血管生成有关。TEM-8是个例外,它被发现与肿瘤的淋巴结转移有相关性。本试验的目的是检测结直肠癌组织中VEGF-D,TEM-8,Podoplanin的含量,并分析其与局部淋巴结转移的相关性。方法用实时半定量PCR方法分析30例结、直肠癌标本中TEM-8,Podoplanin,VEGF-D的表达,并结合病理学分期,淋巴结转移等肿瘤特点进行分析结果VEGF-D,TEM-8,Podoplani水平在肿瘤组织中均较癌旁组织中升高(VEGF-D在肿瘤组织中含量为0.0055±0.0036,癌旁组织中为0.0015±0.0015,P<0.05,podoplanin在肿瘤组织中含量为0.0136±0.0106,癌旁组织中为0.0014±0.0018,P<0.05,TEM-8在肿瘤组织中含量为0.0006±0.0004,癌旁组织中为0.0043±0.0035,P<0.05)。Podoplanin的含量在Dukes B(0.0074±0.0126),DukesC(0.0171±0.0159)样本之间显示显著性差异(P<0.05)。在有淋巴结转移(0.0247±0.0218)和无淋巴结转移(0.00681±0.0108)的样本之间亦显示有明显差异(P<0.05)。VEGF-D的含量在Dukes B(0.00486±0.00186),Dukes C(0.00621±0.00269)样本中未显示明显差异(P>0.05)。在有淋巴结转移(0.00847±0.00218)和无淋巴结转移(0.00481±0.00108)的样本之间则显示有明显差异(P<0.05)。TEM-8在Dukes B,Dukes C样本中的含量分别为0.0028±0.0141,0.00375±0.0162,有显著性差异(P<0.05)。在有淋巴结转移(0.00415±0.0137)和无淋巴结转移(0.00213±0.00584)的样本之间亦显示有明显差异(P<0.05)。结论TEM-8,Podoplanin,VEGF-D均可作为结、直肠癌的标记物,并可反映淋巴结转移状况,TEM-8更可作为肿瘤特异性标记物,在结、直肠癌的诊断,预后判断,治疗中可望发挥重要作用。
【Abstract】 Objectives Lymphatic spread of colorectal cancer cells to regional lymph nodes is one of the early events in metastatic cancers and often is associated with distant metastatic spread and a poor prognosis.In this experiment,the expression levels of lymphatic endothelial markers—VEGF-D,podoplanin,and a newly described tumor marker,TEM-8 were assessed half-quantified by using a real-time PCR(RT-PCR) technique,to evaluate the prognostic value of the three markers in colorectal cancer.methods Tumor tissue and adjacent normal mucosa were obtained from 30 colorectal cancer patients immediately after surgical resection.Levels of VEGF-D, podoplanin and TEM-8 were examined for each sample using real-time RT-PCR.Results VEGF-D,podoplanin,and TEM-8 levels were significantly higher in tumor tissues than that in normal mucosa(VEGF-D level detected in tumor tissue was 0.0055±0.0036,and in normal mucosa,0.0015±0.0015,P<0.05,podoplanin levels in tumor tissues and normal mucosa were 0.0136±0.0106,and 0.0014±0.0018, respectively,P<0.05,and for TEM-8 levels,0.0006±0.0004,in tumor tissues,0.0043±0.0035,in normal mucosa,P<0.05).In the cases with regional lymphnode metastasis,VEGF-D levels were significantly higher than those without(0.00847±0.00218,and 0.00481±0.00108,respectively,P<0.05),but no correlation was found between VEGF-D level and tumor stage(in Dukes B group,VEGF-D level was 0.00486±0.00186,in Dukes C,0.00621±0.00269,P>0.05).While TEM-8(Dukes B,0.0028±0.0141,Dukes C,0.00375±0.0162,P<0.05,lymphnode positive, 0.00415±0.0137,lymphnode negative,0.00213±0.00584,P<0.05) and podoplanin (Dukes B,0.0074±0.0126,Dukes C,0.0171±0.0159,P<0.05,lymphnode positive, 0.0247±0.0218,lymphnode negative,0.00681±0.0108,P<0.05) levels was correlated with both regional node metastasis and tumor stage.Conclusion VEGF-D,Podoplanin and TEM-8 could act as tumor markers for colorectal cancer,and were correlated with regional lymphnode metastasis.TEM-8 was proved to be an tumor spicific marker,may play an important role in the diagnosis,prognostic prediction,and treatment of colorectal cancer.
- 【网络出版投稿人】 中国协和医科大学 【网络出版年期】2009年 07期
- 【分类号】R735.3
- 【下载频次】170