【作者】 李毅清；

【导师】 金毕；

【作者基本信息】 华中科技大学 ， 外科学， 2008， 博士

【摘要】 第一部分丝素蛋白改性聚氨酯复合材料的血液相容性研究目的:研究不同SF配比的改性PU材料人造血管的血液相容性。方法:将PU与SF按照不同配比混合制成SF-PU复合薄膜,与橡胶膜、e-PTFE膜相比较,通过溶血试验、全血凝固时间、体外凝血时间、复钙时间等,探讨不同含量SF对改性PU材料血液相容性的影响,以及SF-PU复合薄膜与橡胶膜、e-PTFE膜材料在血液相容性方面的差异。结果:橡胶膜血液相容性差,e-PTFE和PU血液相容性较好。结论:通过控制SF的含量能进一步提高PU材料的抗凝血性能。第二部分携带阿司匹林的丝素蛋白改性聚氨酯复合材料的物理性能研究目的:研究添加阿司匹林后SF-PU(5∶5)复合材料的主要物理性能。方法:用湿法成膜工艺制备含0%,5%,10%3种不同含量阿司匹林的SF-PU共混膜,对所制备的膜进行膜的形貌电镜观察、力学拉伸测试、红外光谱测试。结果:湿法成膜工艺制得的膜外观、光滑度、拉伸应力、初始模量优良,断裂伸长和亲水性较好。红外测试发现并无新官能团产生,除断裂伸长随阿司匹林含量递增而逐渐减少外,其初始模量,断裂功和断裂强力呈先减少后增加的“U”形变化。结论:阿司匹林含量控制在5%时,可与SF-PU(5∶5)材料混合制成具有优良力学性能的生物膜。第三部分携带阿司匹林的丝素蛋白改性聚氨酯复合材料的组织相容性研究目的:研究添加阿司匹林的SF-PU(5∶5)复合材料的组织相容性。方法:将0%、5%、10%的阿司匹林共混入PU与SF按照5∶5的配比制成的SF-PU(5∶5)复合薄膜。通过大鼠急性毒性实验、肌肉植入局部反应实验、组织切片美兰染色、白细胞及血小板计数以及透射电镜观察,探讨不同含量阿司匹林对SF-PU(5∶5)材料组织相容性的影响。结果:SF-PU(5∶5)混合膜比传统的e-PTFE膜组织相容性好,内附阿司匹林可改善混合膜的抗炎效果,显著增加抗血小板聚集效果,携带5%的阿司匹林的材料组织相容性明显提高,然携带10%的阿司匹林可造成一定的副作用。结论:阿司匹林的含量控制在5%时,可与SF-PU(5∶5)材料混合制成组织相容性比传统的e-PTFE更好的生物膜。更多还原

【Abstract】 Part one:Blood Compatibility of Polyurethane Modified by Silk FibroinObjective:To study the blood compatibility of PU modified by SF with different ratio.Methods:Blend films of SF powder and medical polyurethane(PU) were produced with different ratio.The influence on blood compatibility of PU modified by different ratio of SF was investigated by using hemolysis test,clotting time(CT),recalcification time (RT),PT,APTT.The blood compatibility of the blend films was compared with that of e-PTFE and rubber membrane.Results:The blood compatibility of rubber membrane was the worst,and that of e-PTFE and PU was better.Rational ratio of SF could further improve the anticoagulate potential of PU.Conclusion:At the optimal ratio of SF,the SF-PU mixture has the best blood compatibility.Part two:The Physical Properties of Polyurethane Modified by Silk Fibroin Carrying AspirinObjective:To investigate the major physical properties of PU modified with 50%SF after addition of aspirin.Methods:The wet method was used to prepare 3 different SF-PU(5:5) films carrying aspirin with doses of 0%,5%,10%respectively,and the SF-PU films carrying aspirin were investigated by using SEM,stretching test and IR spectrum. Results:The films prepared by wet method achieved better appearance,smoothness, stretching stress and initial modulus.Infrared test did not detect any new functional group. In terms of mechanical properties,except for the extension at break which was decreased with the increase of aspirin dose,the curve of the initial modulus,the breaking work and the breaking strength showed a "U" shape--decreases at first and increases afterwards.Conclusion:5%dose of aspirin can be mixed with SF-PU(5:5) films to prepare the biomaterial with better mechanical properties.Part three:The Tissue Compatibility of Polyurethane Modified by Silk Fibroin Carrying AspirinObjective:To investigate the tissue compatibility of PU modified by 50%SF after addition of aspirin.Methods:SF and PU were mixed with a ratio of 5 to 5 to prepare SF-PU(5:5) blend films and subsequently aspirin with doses of 0%,5%and 10%was added respectively.By using rat acute toxicity test,test of local reaction in muscle,tissue section staining, WBC and PLT count,and the TEM,the influence of different doses of aspirin on the tissue compatibility of SF-PU(5:5) was studied.Results:The blend film of SF-PU(5:5) had the better tissue compatibility than traditional e-PTFE.The effects of antiinflammation and antiplatelet aggregation of SF-PU (5:5) were improved after addition of different doses of aspirin,especially with the dose of 5%.But 10%aspirin exerted some side-effect.Conclusion:5%dose of aspirin can be mixed with SF-PU(5:5) films to prepare the biomaterial with better tissue compatibility than the traditional e-PTFE.更多还原