【作者】 马勇胜；

【导师】 洪光祥；

【作者基本信息】 华中科技大学 ， 手外科， 2008， 博士

【摘要】 目的:应用大鼠胸腺内注射不同剂量异基因抗原(MHC)的方法,来诱导大鼠对同种异体坐骨神经移植物产生不同完全度的特异性免疫耐受,从剂量学上,研究诱导免疫耐受的量效关系。方法:选用清洁级雄性SD大鼠50只为受体,雄性Wistar大鼠20只为供体,随机分为5组,每组10只:A:新鲜白体神经移植组;B:新鲜异体神经移植组;C:低剂量异基因抗原注射异体神经移植组;D:中剂量异基因抗原注射异体神经移植组;E:高剂量异基因抗原注射异体神经移植组。取供体清洁级雄性Wistar大鼠脾,提取脾细胞悬液,并进行异基因抗原的制备。采用BD针,分别把异体抗原注射受体SD大鼠胸腺内,剂量分别采用C组1.5mg/只,D组3mg/只,E组6mg/只。注射后2周做迟发性超敏反应(DTH)检查,进行同种异体新鲜坐骨神经移植,术后2周分别进行运动神经传导速度检查、病理学检查、透射电镜检查、混合淋巴细胞培养(MLC)和血清sIL—2R检查。结果:运动神经传导速度检测E组(25.56±4.67)m/s与A组接近(p＞0.05),E组优于C组(p＜0.05)。病理学及透射电镜观察E组接近于A组(p＞0.05)优于D组、C组;C组、D组显著优于B组。混合淋巴细胞培养E组(0.392±0.096)优于C组(p＜0.05);A组、C组、D组、E组与B组有显著性差异(p＜0.05)。sIL—2R水平E组(0.117±0.050)、迟发性超敏反应E组(1.34±0.478)mm检查结果优于C组(p＜0.05);A组、C组、D组、E组与B组有显著性差异(p＜0.05)。结论:胸腺内注射异基因抗原可以诱导大鼠对异体坐骨神经移植的免疫耐受,并且注射异基因抗原剂量与诱导的免疫耐受完全度呈正线性关系。目的:探讨胸腺内注射异基因抗原在建立特异性骨移植免疫耐受中的作用。方法:自供体SD大鼠脾细胞中提取MHC抗原,注入受体Wistar大鼠胸腺内,二周后移植供体鼠骨。同时另设四组对照组,即自体骨移植组、异体骨移植组、冷冻后异体骨移植组、应用免疫抑制剂异体骨移植组,术后一.二.四.六周观察切口症状,移植段的X线表现后各组杀死两只鼠取移植段股骨及周围软组织行大体及光镜下检查;及术后四周移植骨超微结构、可溶性白细胞介素—2受体、混合淋巴细胞培养和迟发性超敏反应的检测。结果:实验组优于冷冻组及异体骨移植组,接近于自体骨移植组与应用免疫抑制剂组。结论:胸腺内注射异基因抗原可诱导对异体骨移植的免疫耐受。更多还原

【Abstract】 Objective:To investigate the influence of intrathymical injection with different dose allogenic antigen in induction of sciatic allograft immunotolerance in rats.Methods:Male Sprague Dawley(SD) rats were idvided into 5 groups(ten rats in each group):nerve autograft group,nerve allograft group,low-dose、medial-dose and high-dose MHC antigen injection groups and male Wistar rats(20 rats) were used as transplant donors.Different dose MHC antigen(1.5mg、3mg、6mg/per group) was extracted from Wistar rats splenocytes through destruction,ultrafugation,dialysis and was intrathymically injected into SD recipients rats,a week after the injection,Wistar rats’ scaric transplantation was performed.Two weeks after the surgery,lab-tests were examined:nerve electrophysiology,pathology,sIL-2R,mixed lymphocyte culture and delayed type hypersensitivity.Results:Motor nerve conduction velocity in group E was(25.56±4.67)m/s and there was no significant difference compared with group A(p＞0.05),but better than group C(p＜0.05).The detections in pathology and electron microscope confirmed that group E and A were similar(p＞0.05)and better than group C、D and group C、D better than B(p＜0.05).The mixed lymphocyte culture in group E was(0.392±0.096). sIL-2R levels of group E was(0.117±0.050).The delayed type hypersensitivity in group E was(1.34±0.478)mm.Conclusions:Induce specific immunotolerance’s effect of intrathymical injection of donor MHC antigen could be improved come up with disparate dose used. Objective:To study the effects of inthathymic inoculation allogenic antigen on the establishment of donor specific bone allograft immunotolerance.Methods:MHC antigens extracted from splenic cells of donor SD mice were intrathymically inoculated to recipients Wistar mice,donor bone was transplanted after 2 weeks.Simultaneous-ly,four control groups were used,such as bone autograft group,bone allograft group,bone freeze thawing allograft group and bone allograft in use of immunosuppres- sant group.At 1,2,4,6 weeks postoperatively,2 animals were sacrificed respectively and the femur and its peripheral tissue was harvested for gross and microscopy observation after symptom and X—ray examination.The ultra microstruc- ture of graft.soluble interleukin-2 receptor,mixed lymphocyte culture and delayed-type hypersensitivity were also detected 4 weeks after the procedure.Results:The test group surpassed bone freeze thawing graft group and bone allograft group and approached to bone autograft group and bone allograft in use of immunosuppressant group.Conclusions:The inthathymic inoculation of donor MHC antigen could induce specific immunotolerance of bone allograft.更多还原