【作者】 邹宇；

【导师】 孔维佳；

【作者基本信息】 华中科技大学 ， 耳鼻咽喉科学， 2007， 博士

【摘要】 第一部分单侧迷路切除术后γ-氨基丁酸A受体亚型在大鼠前庭内侧核中的变化目的观察左侧迷路切除术后大鼠前庭内侧核(medial vestibular nuclei, MVN)内γ-氨基丁酸A受体(gamma-aminobutyric acid A receptor, GABAA receptor)α1、β2、γ2亚型的表达变化。方法利用免疫组织化学、原位杂交组织化学、蛋白免疫印记法、RT-PCR的方法,切除大鼠一侧迷路,观察前庭内侧核区GABAA受体α1、β2、γ2亚型表达的变化,及其在前庭代偿中可能的作用。结果各组手术两侧比较及术后1天、3天、7天组与假手术组比较差异均无显著性意义。结论在前庭代偿的早期,GABAA受体α1、β2、γ2亚型表达的变化极可能没有涉及前庭代偿。第二部分单侧迷路切除术后毒蕈碱受体亚型在大鼠前庭内侧核中的变化目的观察左侧迷路切除术后大鼠前庭内侧核(medial vestibular nuclei, MVN)内毒蕈碱受体M1、M3、M5亚型的表达变化。方法利用免疫组织化学、RT-PCR的方法,切除大鼠一侧迷路,观察前庭内侧核区毒蕈碱受体M1、M3、M5亚型表达的变化,及其在前庭代偿中可能的作用。结果单侧迷路切除术(UL)后可诱导双侧MVN区毒蕈碱受体M1、M3、M5亚型减少,术后1天最少,此后3天至7天处于上升趋势,7天时和假手术组比较表达无差异。各组手术两侧比较均无差异。结论UL后可诱导MVN区毒蕈碱受体M1、M3、M5亚型减少。前庭中枢神经元的静息放电降低可能与毒蕈碱受体M1、M3、M5亚型在MVN内减少有关,但其在前庭代偿中的作用尚有待研究?第三部分单侧迷路切除术后毒蕈碱受体亚型在大鼠小脑绒球中的变化目的观察左侧迷路切除术后大鼠小脑绒球内毒蕈碱受体M1、M3、M5亚型的表达变化。方法利用RT-PCR的方法,切除大鼠一侧迷路,观察小脑绒球毒蕈碱受体M1、M3、M5亚型表达的变化,及其在前庭代偿中可能的作用。结果单侧迷路切除术(UL)后可诱导双侧绒球内毒蕈碱受体M1、M3、M5亚型减少,术后1天最少,此后3天至7天处于上升趋势,7天时和假手术组比较表达无差异。各组手术两侧比较均无差异。结论UL后可诱导绒球内毒蕈碱受体M1、M3、M5亚型减少。前庭中枢神经元的静息放电降低可能与毒蕈碱受体M1、M3、M5亚型在绒球内减少有关,但其在前庭代偿中的作用尚有待研究?更多还原

【Abstract】 PartⅠExpression change of GABAA receptor subunits in rat medial vestibular nucleus following unilateral labyrinthectomyObjective To observe the expression of gamma-aminobutyric acid A receptor (GABAA receptor)α1、β2、γ2 subunits in rat medial vestibular nucleus (MVN) following left unilateral labyrinthectomy (UL). Methods The immunohistochemistry, In situ hybridization histochemistry, Western-blot and RT-PCR were used to observe the expression of GABAA receptorα1、β2、γ2 subunits post-unilateral labyrinthectomy and investigate its effect on vestibular compensation. Results No difference were observe in the ipsilateral and contralateral MVN at any group. No difference were observe in the 1 day, 3 day, 7 day than sham operation MVN following UL. Conclusion Our study suggest that expression change of GABAA receptorα1、β2、γ2 subunits in the MVN most probably were not involved in the early stage of the vestibular compensation process. PartⅡExpression change of Muscarinic receptor subnuits in rat medial vestibular nucleus following unilateral labyrinthectomyObjective To observe the expression of Muscarinic receptor M1、M3、M5 subunits in rat medial vestibular nucleus (MVN) following left unilateral labyrinthectomy (UL). Methods The immunohistochemistry and RT-PCR were used to observe the expression of Muscarinic receptor M1、M3、M5 subunits post-unilateral labyrinthectomy and investigate its effect on vestibular compensation. Results Muscarinic receptor M1、M3、M5 subunits were induced decrease in both side MVN after unilateral labyrinthectomy. The least expression in the 1 day MVN of following UL. The expression is rising from the 3 day to 7 day MVN of following Ul. No difference were observe in the 7 day and sham operation MVN following UL. No difference were observe in the ipsilateral and contralateral MVN at any group. Conclusions Muscarinic receptor M1、M3、M5 subunits were induced decrease in the MVN after unilateral labyrinthectomy. The fall in the resting discharge of the central vestibular neurons may be caused by the decrease of Muscarinic receptor M1、M3、M5 subunits in the MVN. But the significance of the change of Muscarinic receptor M1、M3、M5 subunits in the vestibular compensation is still unknown. PartⅢExpression change of Muscarinic receptor subnuits in rat flocculus following unilateral labyrinthectomyObjective To observe the expression of Muscarinic receptor M1、M3、M5 subunits in rat flocculus following left unilateral labyrinthectomy (UL). Methods The RT-PCR was used to observe the expression of Muscarinic receptor M1、M3、M5 subunits post-unilateral labyrinthectomy and investigate its effect on vestibular compensation. Results Muscarinic receptor M1、M3、M5 subunits were induced decrease in both side flocculus after unilateral labyrinthectomy. The least expression in the 1 day flocculus of following UL. The expression is rising from the 3 day to 7 day flocculus of following Ul. No difference were observe in the 7 day and sham operation flocculus following UL. No difference were observe in the ipsilateral and contralateral flocculus at any group. Conclusions Muscarinic receptor M1、M3、M5 subunits were induced decrease in the flocculus after unilateral labyrinthectomy. The fall in the resting discharge of the central vestibular neurons may be caused by the decrease of Muscarinic receptor M1、M3、M5 subunits in the flocculus. But the significance of the change of Muscarinic receptor M1、M3、M5 subunits in the vestibular compensation is still unknown.更多还原