【作者】 付春茂；

【导师】 孙新华；

【作者基本信息】 吉林大学 ， 口腔临床医学， 2008， 博士

【摘要】 本研究将具有抗菌性能的有机配体季铵阳离子和无机阳离子银离子进行合成,形成一种新型的复合抗菌剂(CTS),使之兼具有机抗菌剂的高效性、持续性及无机抗菌剂的安全性、不易产生耐药性。然后通过一种新型的无机填料介孔二氧化硅SBA-15吸附复合抗菌剂,并经表面处理后加入自行研制的光敏树脂粘接剂中。介孔二氧化硅SBA-15可对树脂粘接剂起到增韧作用,利用SBA-15良好的水热稳定性和分子吸附特性能赋予粘接剂缓释抗菌物质的新性能。对合成后的复合抗菌光敏树脂粘接剂,采用X-射线衍射、氮气吸附实验、银离子释放性能测试、抑菌环实验和扫描电镜观察等方法,研究树脂粘接剂的剪切性能,老化性能,抗菌性能与生物相容性。结果显示:吸附CTS的SBA-15颗粒,在树脂中含量为15%的条件下,树脂粘接剂的剪切性能最为理想,达到13.1MPa,能充分满足临床需要。树脂的老化实验表明:吸附CTS的SBA-15含量为0%-15%的树脂粘接剂的剪切强度与断裂面结构于0周和24周时无明显的差异。银离子释放性能和抑菌环实验表明:当吸附CTS的SBA-15含量为10%-20%,8-24周时,银离子长期释放性能无明显差距,复合抗菌树脂粘接剂的抑菌环范围则保持相对稳定。依据常规复合树脂的性能标准及中华人民共和国医药行业管理标准的规定,对所研制的复合抗菌粘接剂的生物学性能进行评价,结果表明研制的复合抗菌粘接剂符合口腔材料生物学性能标准的规定。本研究为国内首次研制的复合抗菌树脂粘接剂。复合抗菌树脂粘接剂在剪切性能上能够满足临床的要求,复合抗菌剂在溶液中缓慢释放能有效抑制变形链球菌的繁殖,并具有良好的生物相容性。复合抗菌树脂粘接剂丰富了口腔粘接剂的种类,为固定矫治器托槽周围釉质脱矿的预防提供一种新思路和新方法。更多还原

【Abstract】 The direct cementation technology for was being unbalanced clinically on has brought enormous convenient, but its negative effect also day by day in relief.Holds around the trough often to occur especially escapes the ore obviously and appears the glaze floccosoids.According to escapes the mechanism its preventing and controlling way which the ore forms to have two:First, the enhancement tooth enamel acid resistance ability, its two, prevented the fungus spot adheres to stick cohere.The enhancement tooth enamel acid resistance ability mainly is the partial use fluoride and contains the fluorine cementation medicinal preparation.The partial use fluorine preparation has the accurate enhancement anti- to escape the ore ability, but its effect must rely on patient’s cooperation.Contains the fluorine cementation medicinal preparation the release fluorine to enter the tooth enamel the ability in to leave in the body experiment to be possible to obtain the confirmation, but the clinical experimental conclusion still could not determine this spot.The tradition prevented the fungus spot adheres to stick cohere the method has the machinery elimination and chemistry preparation application.The use elimination fungus spot method prevented correcting is being unbalanced the patient tooth enamel to escape the ore fixedly to have easy and feasible, the clinical effect accurate characteristic, but this way efficiency is low, also cooperates the dependence to the patient to be big.After consults the domestic and foreign literature, at present includes antibacterial material the cementation medicinal preparation to substitute gradually contains the fluorine cementation medicinal preparation to become is being unbalanced the direction which the cementation medicinal preparation further studies. This research main use has the antibacterial performance to match the body organically and to have the antibacterial performance the inorganic positive ion to form the compound antibiotics, causes it to have at the same time the organic antibiotics effectiveness, long-enduring and the inorganic antibiotics security, not easy to have the drug resistance, and adsorbs the compound antibiotics after one kind of new inorganic padding SBA-15, add-on enters after the surface treatment is being unbalanced in the cementation medicinal preparation, cuts the performance, the antibacterial performance and biological compatibility these aspects to it conducts the research.This research including three aspects:1、compound antibacterial resin cementation medicinal preparation synthesisLies between hole silicon dioxide SBA-15 because has the big relative surface to accumulate with the hole volume aperture one also may adjust the appearance controllable surface easy functional group in a nanometer size and so on a series of characteristics thus has the broad application prospect in the biological macro-molecule separation as well as the nanometer material size and the dimension control aspect, we first the water hot way synthesized had the foreword to lie between hole silica material SBA-15, and designed through the orthogonal experiment further optimizes the experimental craft and the condition, obtained had the aperture to distribute 9.9nm, the relative surface and the hole volume respectively is700m2/gand1.45cm3/g.The structure good also the appearance neat has the foreword to lie between hole oxide compound material SBA-15.Afterwards synthesizes has the compound antibacterial function antibiotics medicinal preparation CTS, and carries on the determination to the CTS antibacterial activeness, CTS is higher than to the distortion chain coccus antibacterial activeness matches body CPC.CTS through heats up - the vacuum adsorption law to assemble CTS to the SBA-15 passageway in, carries on the determination with the atomic absorption law to the loading,1gthe SBA-15 average adsorbs CTS 915.8mg.After SBA-15 silicon hydride coupling medicinal preparation processing joins in the photosensitive resin cementation medicinal preparation by the different proportion which independently develops to make the compound antibacterial resin cementation medicinal preparation.2、compound antibacterial resin cementation medicinal preparation performance testTo the compound antibacterial resin cementation medicinal preparation which develops cuts the performance, the resin based on the conventional compound resin performance standard gets older, antibiotics release performance carries on the appraisal; The result showed that, holds the trough cementation intensity test result to fill in adds decorates after after the coupling to lie between hole SBA-15 to be possible to enhance the resin stretch performance remarkably, increases along with the SBA-15 content, resin system intensity obvious increase, when the SBA-15 content is 15%, the compound resin shearing strength is most greatly 13.1Mpa, may reach the resin matrix 1.45 times. When the SBA-15 content continues the increase, the compound resin shearing strength appears drops obviously, the shearing strength and the domestically produced end product Beijing and Tianjin tooth enamel cementation medicinal preparation and overseas transbond compared somewhat low still slightly. The shearing strength 24 weeks aging experiments demonstration, lengthens the different group along with the time the resin cementation medicinal preparation shearing strength to appear the varying degree to drop, the SBA-15 proportion is 20% resin drops obviously. The electricity mirror structure demonstrated the SBA-15 content is 0%-15% resin fracture structure 0 weeks and 24 week not obvious differences. The SBA-15 content is 20% time 0 week and 24 weeks has the obvious difference. The silver ion release tests demonstrated the compound antibacterial resin cementation medicinal preparation soaks the 2~24 week after the distilled water the silver ion release quantity, soaks when the 2nd week the silver ion release are most, accounts for the 24th week total quantity 30%, after soaks 8 weeks the silver ion release gradually to become stable decides, the SBA-15 content is 10%~20% time compound antibacterial resin cementation medicinal preparation soaks the 8~24 week silver ion release density not obvious difference. Damps the ring experiment to contain not on the same scale the CTS resin cementation medicinal preparation gathers fully when 2 weeks all around the resin all to appear obviously damps the ring, when 8-24 week, the SBA-15 content is around 2.5% resins cementation medicinal preparation damps the ring to reduce obviously, when 24 weeks, damps the ring to vanish nearly. The SBA-15 content is 5% resin cementation medicinal preparation its bacteriostasis scope also lengthens its bacteriostasis scope along with the time also gradually to reduce. The SBA-15 content is 7.5%, 10%, 15%, 20% resin cementation medicinal preparation damps the ring scope to maintain relatively stably.3、compound antibacterial cementation resin biology performance evaluationToxicity test in vitro, acute systemic toxicity test by oral, oral mucosa stimulation test, pulp and dentition applying test, etc show that the resin composites we developed has no cell toxicity, pulp and dentition stimulation, acute systemic toxicity and oral mucosa stimulation as well. This new type of material possesses well biological compatibility.更多还原