【作者】 夏斌；

【导师】 赵忠新； 赵瑛； 周晖；

【作者基本信息】 第二军医大学 ， 神经病学， 2007， 博士

【摘要】 目的评价粘附分子检测在脑梗死临床诊断和治疗中的价值。方法流式细胞仪和酶联免疫吸附法测定颈动脉粥样硬化(CAS)和脑梗死患者外周血白细胞﹑血小板粘附分子的表达,血小板-白细胞凝集性,血清可溶性粘附分子的浓度。免疫组化和原位杂交检测大鼠缺血/再灌注模型内皮细胞粘附分子的表达。结果血小板膜糖蛋白在CAS患者中的表达升高,是CAS发生的独立危险因素;伴脑梗死CAS患者较不伴脑梗死者白细胞﹑血小板粘附分子表达升高,血清可溶性粘附分子浓度增高,血小板-白细胞凝集性增加,粘附分子是CAS患者出现脑梗死的独立危险因素;可溶性细胞间粘附分子-1(sICAM-1)是非栓塞性脑梗死复发的独立危险因素;巴曲酶和通心络治疗涉及到粘附分子。结论粘附分子可作为CAS和脑梗死发病的预测指标和药物治疗的监测指标。更多还原

【Abstract】 Objective To evaluate the value of detection adhesion molecules for the diagnosis and treatment of cerebral infarction. Methods By flow cytometry and enzyme-lined immunosorbent assay,the expression of adhesion molecule on leukocyte﹑platelets,platelet-leukocyte aggregates and the concentrations of soluble adhesion molecule from peripheral blood were measured in patients with carotid atherosclerosis(CAS)and cerebral infarction. The expression of adhesion molecule on endothelial cell were detected by immunohistochemistry and in situ hybridization in rat cerebral ischemia/reperfusion models. Results The expression of platelet membrance glycoprotein was increased in patients with CAS and it was an independent risk factor in CAS.The expression of adhesion mocules on leukocyte﹑platelets,platelet-leukocyte aggregates and the concentrations of soluble forms were elevated in CAS with infarction patients compared to without infarction.Adhesion molecule was an independent risk factor in CAS occurring cerebral infarction.Soluble intercellular adhesion molecule-1(sICAM-1) was a independent risk factors in nonembolic ischemic stroke recurrent.The treatment of bartroxobin and Tong Xin Luo involved in adhesion molecules.Conclusions Adhesion molecules may as a index of predict and treatment monitoring for cerebral infarction.更多还原