【作者】 史仍飞；

【导师】 郭俊生；

【作者基本信息】 第二军医大学 ， 军事预防医学， 2007， 博士

【摘要】 【目的】研究不同模式振动训练对大鼠运动能力的影响,探讨振动训练提高运动能力的细胞分子生物学机制。【方法】以42只雄性SD大鼠为实验对象,建立不同振动训练模型,评价各实验组大鼠骨骼肌运动能力变化,测试肌组织细胞水平、肌肉肌酸激酶(CK)、肌细胞机械生长因子(Mechano growth factor, MGF)表达水平。通过肌卫星细胞培养,测试机械刺激对卫星细胞增殖和蛋白含量影响。【结果】中频率长时间振动组(ML)大鼠游泳成绩和最大抓力均显著高于对照组(CC),且该组的腓肠肌相对质量、肌纤维横截面积(CSA)、CK和MGF mRNA的表达均显著增加(P < 0.05);卫星细胞培养结果显示,机械张力刺激后,卫星细胞增殖能力均大于对照组(p< 0.01),且15%拉伸刺激增殖效果最佳。【结论】振动训练刺激可能通过激活卫星细胞增殖和肌细胞机械生长因子的表达、促进肌纤维选择性肥大、提高骨骼肌细胞代谢酶CK活性等途径提高肌肉的运动能力。更多还原

【Abstract】 Objective: The present study was designed to examine the effects of different vibration trainings on the exercise capacity, and further explore the underlying mechanism of cellular and molecular biology. Methods: 42 male Sprague-Dawley rats were used to establish different vibration training models. Exercise capacity in rat skeletal muscle was evaluated, the chemical levels in muscle tissue, muscle creatine kinase (CK) and myocytes Mechano growth factor (MGF) expression levels were tested. Proliferation and protein content of satellite stimulated by vibration were detected through muscle myo-satellite cell culture experiments. Results: Compared to the control group(CC), scores of swimming and grasping power in the medium-frequency vibration prolonged group(ML) were significantly improved. In addition, the relative quality of the gastrocnemius muscle, fiber cross-sectional area, CK and MGF mRNA expression was significantly increased in ML group. Cultured quiescent satellite cells were subjected to mechanical stretch in a Flexer Cell System. In response to stretch stimulation, the multiplication ability and protein content in satellite cell were higher than those in the control group. Conclusions: Vibration training may stimulate proliferation of satellite cells and the expression of MGF mRNA, promote muscle hypertrophy and CK enzymes activity, then lead to the enhancement of exercise performance.更多还原