【作者】 姚凤祯；

【导师】 孙申田；

【作者基本信息】 黑龙江中医药大学 ， 针灸推拿学， 2007， 博士

【摘要】 目的：通过研究针刺家兔内关穴对心律失常的治疗作用，并从分子生物学水平探讨其作用机制，为针刺治疗心律失常提供理论依据。方法：采用静脉注射盐酸肾上腺素的方法复制快速型心律失常家兔模型，将家兔随机分为5组：空白对照组、模型对照组、针刺预防组、针刺治疗组和生脉注射液组；采用静脉注射盐酸维拉帕米的方法复制缓慢型心律失常家兔模型，将家兔随机分为5组：空白对照组、模型对照组、针刺预防组、针刺治疗组、参附注射液组。运用电生理学、RT-PCR法及分光光度法等技术手段，观察针刺对心律失常家兔模型心电时相变化、快速型心律失常家兔模型心肌细胞离子通道的表达和缓慢型心律失常家兔模型心肌钠-钾-ATP酶的影响。结果：针刺治疗组、生脉注射液组与模型对照组比较，快速型心律失常出现的时间及持续时间均缩短，心肌细胞离子通道表达增强，差异有统计学意义；针刺治疗组与生脉注射液组相比，心律失常出现时间、持续时间及心肌细胞离子通道表达的差异均无统计学意义。模型对照组与其它组相比，缓慢型心律失常出现的时间及持续时间均有延长，差异有统计学意义；空白组与其它组相比，心肌组织Na~+-K~+-ATPase活性升高，差异有统计学意义；针刺预防组、针刺治疗组、参附注射液组与模型组相比较，心肌组织Na~+-K~+-ATPase活性提高，差异有统计学意义，且针刺预防组心肌组织Na~+-K~+-ATPase活性较针刺治疗组及参附注射液组明显升高。结论：针刺内关穴可明显延缓家兔模型快速型及缓慢型心律失常出现的时间，缩短其恢复时间；针刺内关穴能够阻断快速型心律失常模型心肌细胞离子通道，并能提高缓慢型心律失常家兔模型心肌钠-钾-ATP酶的活性，这可能是其防治心律失常的机制之一。更多还原

【Abstract】 Objective: Studying the effect of curing arrhythmia by acupuncture Neiguan point of rabbit and discussing its mechanism, to provide a theoretic basis for this therapy.Methods: Dividing 40 rabbits into 5 groups randomly, there are 8 rabbits in each group. They are the normal control group, the model control group, the acupuncture prevent group, the acupuncture treat group and shengmai injection control group. Except the normal control group, replicated the animal models-rabbits of fast arrhythmia by quickly intravenous injecting adrenaline. Dividing 40 rabbits into 5 groups randomly, there are 8 rabbits in each group.They are the normal control group, the model control group, the acupuncture prevent group, the acupuncture treat group and Shenfu injection control group.Except the normal control group, replicated the animal models-rabbits of chronic arrhythmia by injecting Verapamil hydrochloride into ear vein.To observe the effects of acupuncture to electrocardiography time of arrhythmia, the current of ionic channels of heart cells of fast arrhythmia and Na~+-K~+-ATP ase activity of chronic arrhythmia, by the way of electrophysiology, RT-PCR and spectrophotometry.Results: Contrasted with the model control group, the starting time and lasting time of fast arrhythmia of the acupuncture treat group and shengmai injection control group was shortened(P<0.05); the current of ionic channels of heart cells descended(P<0.05).The differences of above indexes between the acupuncture treat group and shengmai injection control group don’t have statistics meaning.Contrasted with other groups, the starting time and lasting time of chronic arrhythmia in the model control group was prolonged(P<0.05); Contrasted with other groups, Na~+-K~+-ATP ase activity of heart muscle cells in the normal control group was increased(P<0.05); Contrasted with the model control group, Na~+-K~+-ATP ase activity in the acupuncture prevent group, the acupuncture treat group and Shenfu injection control group was increased(P<0.05); Even contrasted with the acupuncture treat group and Shenfu injection control group, Na~+-K~+-ATP ase activity in the acupuncture prevent group was evidently increased.Conclusion: The therapy of acupuncture Neiguan point can evidently delay the starting time of experimental fast arrhythmia and chronic arrhythmia of rabbits, shorten its resuming time. The therapy also can cut off the ionic channels of heart cells of fast arrhythmia and increase Na~+-K~+-ATP ase activity of heart cells of chronic arrhythmia. It maybe one of its mechanisms of preventing and treating chronic arrhythmia.更多还原