【作者】 李竹英；

【导师】 刘建秋； 孙伟正；

【作者基本信息】 黑龙江中医药大学 ， 中西医结合临床， 2007， 博士

【摘要】 目的：观察平喘Ⅰ号对哮喘大鼠模型信号转导子和转录激活子(STAT6)蛋白含量，嗜酸性细胞特异性趋化因子(Eotaxin)蛋白含量及其mRNA表达以及肺泡灌洗液(BALF)和血清中IL-4，IL-13含量及EOS数的影响，探讨平喘Ⅰ号对支气管哮喘的作用机理。方法：采用免疫组化法和原位杂交法检测哮喘大鼠模型STAT6，Eotaxin蛋白及其mRNA表达；酶联免疫吸附试验法(ELISA法)检测大鼠肺泡灌洗液及血清中IL-4，IL-13含量；Wright(瑞士)染色法测定BALF中WBC数，EOS数及分类。结果：1、哮喘大鼠模型组STAT6蛋白，Eotaxin蛋白及其mRNA表达明显高于正常对照组，经统计学处理有显著差异(P＜0.01)，中药低、中、高剂量组STAT6蛋白，Eotaxin蛋白含量低于模型组，其mRNA表达明显弱于模型组，经统计学处理，有显著差异(P＜0.01)，中药中、高剂量组与地塞米松组相近，无显著差异(P＞0.05)。2、模型组支气管肺泡灌洗液及血清中IL-4、IL-13含量明显高于正常对照组，经统计学处理，有显著差异(P＜0.01)，中药低、中、高剂量组BALF及血清IL-4，IL-13含量明显低于模型组，有显著差异(P＜0.01)，中、高剂量组与地塞米松组相近，无显著差异(P＞0.05)。3、哮喘模型组BALF中EOS绝对值及EOS％较正常对照组显著升高，经统计学处理有显著差异(P＜0.01)，中药低、中、高剂量组BALF中EOS绝对值及EOS％明显低于模型组，有显著差异(P＜0.01)，中、高剂量组与地塞米松组相近，无显著差异(P＞0.05)。结论：平喘Ⅰ号通过降低哮喘大鼠模型STAT6蛋白、Eotaxin蛋白含量以及减弱STAT6 mRNA、Eotaxin mRNA在气道上皮细胞的表达，降低哮喘大鼠支气管肺泡灌洗液及血清中IL-4、IL-13含量，降低BALF中EOS数，来干预气道炎症，从而达到治疗支气管哮喘的目的。更多还原

【Abstract】 Objective:Observe the influence of Chinese herbal medicine (PingChuan I ) to the asthma rat model STAT6 protein content, Eotaxin protein content and their mRNA and the contents IL-4、IL-13 and EOS numbers in the serum and BALF, inquiry into the function mechanism of PingChuan I to bronchus asthma.Method:Adopting immunohistochemical method and hybridization in situ method examination asthma rat model the expression of STAT6, Eotaxin protein and their mRNA; adopting enzyme linked immunosorbent assay(ELISA) to examine the contents IL-4、IL-13 in BALF and serum of rat, The Wright staining examine the WBC number、classification and EOS number in the BALF.Result:1.The asthma rat model group’s STAT6 protein, Eotaxin protein and their mRNA’s expression are obviously higher than the normal matched control, learned by covariance examination with obvious difference (P<0.01). Chinese herbal medicine low doses group, medium doses group and high doses group’s the contents of STAT6 protein, Eotaxin protein is lower than model group, and their mRNA expression obviously weaker than model group, learned by covariance examination with obvious difference (P<0.01), but medium doses group, high doses group are close by dexamethasone group, no obvious difference (P>0.05).2. Model set’s contents of IL-4,IL-13 in BALF and serum are obviously higher than normal matched control, learned by covariance examination with obvious difference(P<0.01). Chinese herbal medicine low doses group, medium doses group and high doses group’s the contents of IL-4, IL-13 in BALF and serum are lower than that model group, but medium doses group, high doses group are close by dexamethasone group, no obvious difference (P>0.05)3. Asthma model group’s #EOS and EOS% in BALF are obviously higher than normal matched control group, learned by covariance examination with obvious difference(P<0.01). Chinese herbal medicine low doses group, medium doses group and high doses group’s #EOS and EOS% in BALF are obviously lower than model group’s, learned by covariance examination with obvious difference(P<0.01), but medium doses group, high doses group are close by dexamethasone group, no obvious difference (P>0.05). Conclusion: The Chinese herbal medicine (PingChuan I ) may lower the contents of STAT6 protein and Eotaxin protein, and die down the expression of STAT6 mRNAs, Eotaxin mRNA in these asthma rats’s airway epithelial cells, lower the contents of IL-4, IL-13 in the asthma rat’s BALF and serum, lower the number of EOS in the BALF, tamper airway’s inflammation, achieve the treatment purpose for the bronchus asthma.更多还原