【作者】 戴林；

【导师】 张学庸； 惠宏襄；

【作者基本信息】 第四军医大学 ， 内科学， 1996， 博士

【摘要】 肿瘤最基本的生物学特征之一是恶性增殖，其增殖过程又与肿瘤细胞浸润、转移及肿瘤患者的复发和预后密切相关，其实质是基因及基因调控异常。目前研究发现，与胃癌相关的癌基因、抑癌基因已十余种之多，如p53突变、c-myc、c-erb-B扩增和H-ras点突变等在胃癌中相当常见。尽管这些异常基因对细胞的作用方式不同，作用途径和作用部位有别，但最终结果都将导致细胞恶性增殖以致癌变。因此，有效地遏制肿瘤细胞增殖是肿瘤药物治疗乃至基因治疗的基础和重要内容。 增殖细胞核抗原(Proliferating cell nuclear antigen，PCNA)已被证实更多还原

【Abstract】 Malignant proliferation is one of the most fundamental biologic behavior of all tumors, and this proliferative process is correlated with cancer cell infiltration, metastatic potential, recurrence as well as prognosis. Recent investigations have shown that many kinds of oncogenes and suppresser genes are involved in gastric carcinogenesis, p53 mutation, c-myc, c-erbB-2 amplification and H-ras point mutation have been reported as having a relatively high occurrence. All these genetic alterations result in the cell malignant proliferation even though they play different roles incontrolling the growth of cancerous and normal cells. Consequently, effective suppression of cell proliferation is the crucial measure of tumor therapy.Proliferating cell nuclear antigen (PCNA) was recently identified as an auxiliary protein of DNA polymerase. It is closely associated with sites of DNA replication and cell’s proliferation states. PCNA is one of the intracellular factors and is common to all /pathways of DNA synthesis. Many previous studies have shown that high expression of PCNA is related to tumorigenesis, metastasis and recurrence.Gene therapy is one of the most important advance in medical biological field in nineties of this century. Antisense technology is regarded as the most promising procedure in gene therapy for cancer. Expression of oncogene may be blocked by antisense .更多还原