【作者】 贾赤宇；

【导师】 陈璧； 王德文； Gerge W.Cherry；

【作者基本信息】 第四军医大学 ， 烧伤外科， 1997， 博士

【摘要】 烧伤是一种常见病，其创面处理贯穿于治疗的全部始终。创面愈合的速度和质量是评价治疗水平高低的唯一可靠指标。由于目前对烧伤创面愈合和后期瘢痕形成的机理及其影响规律缺乏确切、深入的研究，因此在一定程度上影响了烧伤治疗水平的进一步提高。烧伤不仅给病人造成极大的肉体和精神上的痛苦，也给社会及家庭带来巨大的经济负担。多年来，国内外虽然有不少学者在此领域进行了大量的研究，但在加速创面愈合及有效控制后期瘢痕形成方面至今尚无令人满意的进展。 为了克服以往烫伤模型制作上的不足，研制了新的较为理想的仪器-恒温恒压电烫仪。在此基础上，分别用小鼠和大鼠制作了不同深度的烫伤模型。发现在温度80℃，压力=0.5Kg的条件下，造成小鼠浅Ⅱ°、深Ⅱ°和Ⅲ°烫伤分别需8秒、10秒和12秒。而在大鼠上造成相同深度的烫伤则在温度=80℃，压力=1.0Kg条件下，需时6秒、9秒和12秒。而大鼠的皮肤较小鼠皮肤厚的多，说明压力在接触性烫伤的致伤因素中作用很大；同时也验证了该仪器具有安全、准确、快速、重复性好，容易操作等优点。 利用基因重组技术，将全长序列的hTGFβ₁构建至能直接在骨骼肌细胞中进行基因表达的特殊载体PCMV₄中，得到新的真核表达载体PCMV₄-TGFβ₁。将其裸DNA直接注入Ⅱ°烫伤大鼠体内，用以探讨基因治疗在促进烧伤创面愈合和控制后期瘢痕形成中的作用，探索其作为核酸疫苗的可行性，为临床治疗寻求新的突破口。即用xba Ⅰ和Hind Ⅲ双酶切hTGFβ₁ cDNA全长序列，回收获得1.36Kb的片断。用相同的酶双酶切更多还原

【Abstract】 Burn is a kind of common illness, management of whose surface of wound should span the whole course of treatment. The only reliable criterion for assessing the level of treatment is the speed and quality of healing of wound. Due to inadequacy in study of the mechanism of healing of burn and scarring that follows and inadequacy in study of the contributing factors, further elevation of the level of treatment of burn is delayed to some extent. Burn not only makes patients physically and mentally suffer tremendously, also imposes a heavy economic burden on their families and society. Scholars at home and abroad have done a lot of work in recent years, but to date no satisfactory development has been achieved yet in speeding up of healing of the wound and effective control of scarring that follows.To overcome the shortcomings in preparation of scald model, a new ideal instrument, constant-temperature constant-pressure electric scalding device, is developed. Using this device, models of scald of varying degree were prepared on mice and rats. It was found that, under conditions of 80℃ in temperature and 0.5Kg in pressure, the times required for creating superficial second-degree scald, deep second-更多还原