【作者】 徐凌；

【导师】 梁星；

【作者基本信息】 四川大学 ， 口腔临床医学， 2006， 博士

【摘要】 随着口腔种植学的理论不断完善和技术不断发展，使得骨内种植体在临床上得以广泛应用。但是，口腔种植修复也存在着一些亟待解决的问题，例如，如何促进种植体与骨之间形成骨整合，使其能尽快完成种植体上部结构的修复，实现义齿的功能修复等。运用生长因子解决这类问题是目前口腔种植修复学领域研究的热点。成骨生长肽(Osteogenic Growth Peptide，OGP)是近年来发现的一种新的促进有丝分裂的生长因子，已有的研究初步证实了其在成骨和造血等方面具有潜在的应用价值。但是，目前对成骨生长肽的研究尚未涉及其对成骨细胞分化作用机制的研究，同时其对骨髓基质细胞增殖分化方面的研究，国内外也未见报道。 本课题的研究目的是探讨OGP对成骨细胞和骨髓基质细胞增殖分化功能的影响，初步阐明其促进成骨的作用机制。实验采用成骨细胞和骨髓基质细胞体外培养模型，应用MTT法和流式细胞仪检测不同浓度的OGP对成骨细胞和骨髓基质细胞增殖率和增殖周期的影响。同时应用酶联免疫检测法、免疫细胞化学染色法、激光共聚焦显微镜、透射电子显微镜、半定量逆转录聚合酶链反应(RT-PCR)等方法检测不同浓度的OGP对成骨更多还原

【Abstract】 Endosteal implant has been widely applied, with the progress of theory and technique of oral implant. But, there have been some pro- blems needing to resolve. For example, how to promote osseointegra- tion between implant and bone around it for completing upper restora- tion to achieve function as soon as possibly? It has been hot region of study in oral implant that utilize growth factors to resolve these pro- blems. People found osteogenic growth peptide as a new mitosis factor can promote bone and blood formation.The purpose of our research is to study the effect of osteogenic growth peptide on osteoblast and marrow stromal cells in proliferation and differentiation and elucidate the mechanism of promotion. In our study, we analyzed the effect of different concentration of OGP on the proliferation of osteobalsts and bone marrow stromal cells using MTT and flow cytomitric analysis, utilizing these models of osteoblast and bone marrow stromal cells cultured in vitro. And we studied the influence of different concentration of OGP on the differentiation of osteoblasts and bone marrow stromal cells using更多还原