【作者】 王大庆；

【导师】 罗清礼；

【作者基本信息】 四川大学 ， 眼科学， 2006， 博士

【摘要】 【研究目的】(1)研究在眼睑基底细胞癌(Basal Cell Carcinoma,BCC)中NES1基因和蛋白的表达情况，并分析其表达与表型之间的关系，从基因和蛋白水平上研究NES1在BCC的发生发展过程中的作用，以期判断NES1基因是否能作为眼睑肿瘤早期诊断和预后判断的标记物；(2)研究NES1基因外显子3的甲基化情况，以期找到NES1在眼睑BCC中异常表达的可能机制，为眼睑BCC的治疗寻找新的靶点。 【研究方法】本实验共分两大部分：第一部分，我们采用实时荧光定量PCR(Real-time Quantitative PCR)和免疫组化的方法检测了NES1在31例眼睑BCC中的基因表达和蛋白(human kallikrein 10,hk10)的表达情况，并分析其表达与表型之间的关系，我们采用Image-Pro Plus5.0图像分析系统对免疫组化结果进行扫描，定量分析了hk10在不同组眼睑BCC中表达；第二部分，采用甲基化特异性PCR(Methylation Specific更多还原

【Abstract】 Purpose (1)To investigate the expression of NES1 gene and protein in eyelid basal cell carcinoma (BCC),and analyze the correlation between its expression and the tumorigenic phenotype. In addition, to study the role of NES1 in oncogenesis and progression of eyelid basal cell carcinoma at gene and protein level, and estimate the feasibility as a tumor marker in the early diagnosis and prognosis.(2) In order to find out the mechanisms of loss of NES1 expression, we have studied the methylation condition in exon 3,and expect to find out a new target in the treatment of eyelid BCC.Methods The experiment has two parts. In the first part, we have studied the expression of NES1 gene and protein in 31 cases of eyelid basal cell carcinoma by using real-time quantitative PCR and immunohistochemistry. and analyzed the correlation between its expression and the tumorigenic phenotype at gene and protein level. We quantificationally analyzed the expression of hk10 in different groups by using Image-Pro Plus5.0 soft. In更多还原