【作者】 盘如刚；

【导师】 陈晓明；

【作者基本信息】 四川大学 ， 眼科学， 2006， 博士

【摘要】 研究背景： 青光眼是一种严重致盲性眼病，降低眼内压是青光眼治疗的一个主要措施，滤过性手术具有较好的降压效果，目前被认为是控制眼压最有效的方法，然而由于创伤修复愈合，滤过口部位成纤维细胞增殖和胶原形成，可导致滤过通道闭合，严重影响青光眼滤过性手术的成功率。针对青光眼手术后瘢痕的形成，目前主要的对抗手段是术中术后采用抗瘢痕药物，临床上应用较广泛的是丝裂霉素C(MMC)和5-氟尿嘧啶(5-FU)，这两种抗代谢药物的应用显著地提高了青光眼滤过性手术的成功率，但是也不可避免损伤邻近眼部组织，对结膜、角膜、巩膜、睫状体、小梁网等组织都有一定的毒副作用。寻找一种疗效高、特异性强、毒副作用小的抗瘢痕药物已成为近年来青光眼研究的热点。 热休克蛋白47(HSP47)是一种分子量为47KD的蛋白，在内质网中能与多种类型胶原和前胶原特异性结合，HSP47作为胶原合成有关的“分子伴侣”，参与前胶原在内质网中的折叠、装配、修饰和转运等过程，对胶原分子合成质量控制起重要的作用，能够防止错误构型的前胶原分泌出内质网。HSP47在创伤修复过程中表达增强，且在全身部分纤维化更多还原

【Abstract】 Backgrouds: Glaucoma is a severe blind leading disease .Reducing the intraocular pressure (IOP) is the main therapeutic measure . Glaucoma filtration surgery (GFS)is the most popular strategy applied to reduce the IOP. However, Fibroproliferating and collagen formation resulted from the fibroblasts lead to the obstruction of the created fistula and subconjunctival filtration area. Antifibrotic therapy is the main means to prevent scarring .Antifibrotics, such as 5-fluorouracil and mitomycin C, are widespread clinically applied to increase the success of surgery.Despite the positive effect on the success rate, diffusion into adjacent ocular tissues may result in impairment of other than the targeted cells and in complications of conjunctiva, cornea, sclera, ciliary body and trabecular meshwork. New strategies are being developed, to find a more effective and less complication way to suppress the wound-healing process.Heat shock protein47(HSP47) is a 47-kDa stress protein that is a collagen-specific molecular chaperone residing in the endoplasmic reticulum (ER). HSP47 is closely involved in the folding, assembly, modification and transport of procollagen. HSP47 might be involved in a quanlity control更多还原