【作者】 杨开明；

【导师】 羊惠君；

【作者基本信息】 四川大学 ， 人体解剖学与组织胚胎学， 2006， 博士

【摘要】 目的 探讨胰腺发育中胰岛细胞的分化、迁移和胰岛的形成，以及在此过程中相关基因表达的变化。对胰岛β细胞损伤后的胰岛再生机制进行初步研究，并检测了β细胞损伤后调控胚胎胰岛细胞分化发育的部分关键性基因Pdx-1、Ngn3、Nkx2.2及Nkx6.1的表达情况，为应用胰腺干细胞作为糖尿病生物治疗性手段提供基础性研究。 材料和方法 1．收集征得孕妇签字同意后、因意外等各方面原因必须终止妊娠的不同胎龄段的正常胚胎标本进行组织学观察，并通过免疫组织化学方法观察胰岛细胞的分化发育和CD34、AFP、IGF-I、IGF-IR，TGF-β、TGF-βR，bFGF及FGFR因子在胰腺发育中的表达分布。 2．采用β细胞毒性剂—Alloxan腹腔注射破坏新生SD大鼠的β细胞，复制糖尿病模型，采用免疫组织化学方法观察胰岛β细胞的再生，并通过RT-PCR、Western-blot方法检测Pdx-1、Ngn3、Nkx2.2及Nkx6.1基因的表达。 结果 1．人胚胎观察 ① 4周时，人胚胰芽由前肠末端发出，腹、背胰在胎龄5周时融更多还原

【Abstract】 ObjectiveTo clarify the differentiation and migration of islet cells as well as islet forming in pancreatic genesis and development, and the expressing changes of related genes in pancreatic development. Meanwhile, Initial investigation were established for mechanism of islets regeneration after β-cells injured. Part vital genes related to islet cells differentiation and development of embryo such as Pdx-1, Ngn3, Nkx2.2 and Nkx6.1 were detected in islets regeneration. It expected to provide elementary investigation for the biological application to finally cure diabetes mellitus. Material & MethodsSample collection was conducted in accordance to the principle of informed consent. Histological method and immunohistological methods were adopted in studying normal embryonic pancreas of donators at 3-14 weeks of gestation, and analysis were made on vital cytokines expression of CD34, AFP, IGF-I, IGF-IR, TGF-β, TGF-βR, bFGF and FGFR for更多还原