【作者】 刘世霆；

【导师】 陈志良；

【作者基本信息】 第一军医大学 ， 药剂学， 2006， 博士

【摘要】 随着外科技术的迅速发展，人们要求影像学能检出更小的病变，更准确地定性，使得磁共振造影剂的研究备受关注。SPIO（超顺磁氧化铁）作为目前磁共振造影剂的研究热点，具有肝脏特异性，即主要由肝脏正常组织中的枯否氏细胞吞噬，而肿瘤组织由于缺乏该类细胞，使得SPIO在肿瘤部位含量低，从而可通过磁共振检查将正常与病变组织区分出来。SPIO在肝脏局灶性病变尤其是小肝癌、微小肝癌的检出率、定性鉴别诊断方面，开创了细胞功能与影像结合的新天地，使得肝脏疾病诊断程序变得更为简单、迅捷。此外，USPIO（超细顺磁氧化铁）造影剂在受体等介导的磁标记探针成像的应用前景也引起人们的广泛注意。所谓磁标记探针是指将顺磁性粒子以某种方式与特异性受体、抗体或基因结合转入细胞内，由于该顺磁性物质在细胞内的蓄积与所连接的物质数量成正比，因此，顺磁性物质引起的MR信号分布代表了受体、抗体或基因的分布，而通过顺磁性标记的配体，在受体介导下，产生特异性浓集，达到选择性强化进而成像的目的。我们的目的是通过详细考察各种因素对形成稳定的纳米Fe₃O₄磁流体的影响，设计合适的制备方案，制备出适合粒径范围的SPIO或USPIO，在活体及细胞水平检验其造影增强效果，并制订相应的质量标准和制各工艺，为工业化生产提供技术支持。 方法与内容：更多还原

【Abstract】 With the rapid development of surgery, we want to get the better result from MR imaging, for example to find the disease with less area, and more accurately. It is more and more important to do some research on MR imaging contrast agents. The SPIO (superparamagnetic iron oxide) MR contrast agents have taken an important part in this field. This is partly because of its liver specification. The main part of SPIO is licked up by Kuffe’s cells in normal liver. The tumor didn’t contain these cells so SPIO concentration was lower in it. The difference between the normal and tumor can be detected in MR imaging. It brought about the new develop of diagnosis in combination cell fuction with MR imaging in liver limit cancer such as little and micro tumors. It was easier and rapider for the diagnosis procedure in liver disease. Besides this, USPIO (Ultrasmall Superparamagnetic Iron Oxide) contrast agents had a very potent future in so called magnetic labeled probe MR imaging. This probe would combine in some way with specific receptor, antibody and gene and go into the cell. The concentration of the magnetic cores inside the cell correlate with the material they could bind with. So the MR imaging signal represent the distribution of the receipt, antibody and gene. What we want to is to study several important factors in更多还原