【作者】 董晓静；

【导师】 胡丽娜；

【作者基本信息】 重庆医科大学 ， 妇产科学， 2006， 博士

【摘要】 陡脉冲电场对细胞膜的不可逆性电击穿导致的细胞死亡成为了恶性肿瘤低创或微创物理治癌的新方式,而其对机体的安全性以及对周边正常组织有否影响是保证其安全治疗的前提。细胞膜作为生物膜在维持细胞稳定性中具有重要的作用。细胞膜电位是作为细胞膜最重要的生物物理特性之一,它在细胞的正常生命活动中亦起着重要的作用,调节着许多重要的细胞功能。膜电位的变化必然会改变细胞膜离子通道状态,导致细胞内离子浓度变化。而离子浓度的改变还可能影响细胞缝隙连接功能,影响肿瘤细胞的增殖和活性。因此本研究拟通过三个部分的研究进行陡脉冲对细胞膜功能的影响及其对机体的安全性作深入的探讨,对陡脉冲研制中某些关键问题作定性及定量的研究。第一部分能量可控陡脉冲对细胞内钙离子浓度及膜电位的影响目的:探讨不同剂量陡脉冲对细胞内游离Ca²⁺浓度([Ca²⁺]i)和细胞膜电位的影响,为进一步研究其治疗恶性肿瘤的机制奠定基础。方法:将乳腺癌细胞MDA-MB-231分为对照组和5个不同剂量ECSP处理组,分别用荧光探针Fluo-3/AM标记Ca²⁺、DiBAC4（3）标更多还原

【Abstract】 The steep pulse which electric field can make the cell death by destroying the cell membrane with irreversible electroporation is a new low and micro trauma method in the treatment of malignant tumor. The safety and the influence of steep pulse on surrounding normal tissue are the prerequisite of it in the treatment. The cell membrane as a biomembrane has very important role in maintaining the stability of cell. The cell membrane potential is one of the most important biophysical characteristics in cell membrane. It has very important function in the cellular normal vital movement, regulating many important cell functions. The changes of cell membrane potential certainly alter the states of ion channel in cellular membrane, which induce the changes of ionic concentration. The changes of ionic concentration may influence the function of intercellular gap junction, which affects the proliferation and activity of tumor cells. So in this succession, we plan to further investigate the influence of ECSP on cellular membrane function and its safety on transplanted tumor rabbit, also give qualitation and quantitation analysis in some key issues concerning the development of ECSP.更多还原