针灸化瘀通络法对痛风性关节炎及高尿酸血症影响的实验研究

【作者】 李彤；

【导师】 黄迪君；

【作者基本信息】 成都中医药大学 ， 针灸推拿学， 2006， 博士

【摘要】 目的：以实验性急性痛风性关节炎模型和高尿酸血症模型动物为研究对象，以中医针灸理论为指导，运用针灸化瘀通络法治疗实验性急性痛风性关节炎和高尿酸血症，初步揭示针灸化瘀通络法的疗效和作用机理，为临床治疗提供科学依据。 方法：采用向大鼠踝关节腔注入尿酸钠溶液形成急性痛风性关节炎作为模型动物，分别采用酵母膏和氧嗪酸钾盐对小鼠灌胃和腹腔注射形成高尿酸血症模型动物，将针灸化瘀通络法分别用于两种模型的治疗，并与抗炎药(消炎痛)和降尿酸药(苯溴马隆和别嘌呤醇)作组间对照，观察其对疼痛、炎症相关指标和对血尿酸水平的影响。 结果：1、通过鼠耳肿胀法以及扭体法、热板法的实验，证明了针灸化瘀通络法具有明显的抗炎镇痛效果(p＜0.05)。2、在急性痛风性关节炎模型动物中，分别通过对局部组织细胞因子、疼痛介质水平的测定，证明了针灸化瘀通络法对急性痛风性关节炎模型动物细胞因子和疼痛介质具有抑制作用(p＜0.05)；通过光镜及电镜观察病理组织，从病理形态学角度来证明实验性急性痛风性关节炎模型的成功和针灸化瘀通络法治疗痛风性关节炎的确切作用。3、针灸化瘀通络法能明显降低异常升高的血尿酸和尿素氮，对黄嘌呤氧化酶的活性有抑制影响(p＜0.05)。 结论：1、针灸化瘀通络法抗炎镇痛效果明显，抗炎作用可能主要与抑制细胞因子释放有关，而镇痛作用可能主要与抑制外周K~+、NE、DA和5-HT释放有关。2、针灸化瘀通络法有抑制XOD的活性，减少尿酸生成和促进尿酸排泄的作用。更多还原

【Abstract】 Objective: Taking the experimental acute gouty arthritis model animal and thehyperuricaemia model animal as the research object, and taking the TCM acupuncture and moxibustion theory as the instruction, using the treatment law of removing blood stasis and dredging collateral of the acupuncture and moxibustion to experimental acute gouty arthritis and the hyperuricaemia, promulgates the curative effect and the functional mechanism of removing blood stasis and dredging collateral of the acupuncture and moxibustion, and provides the scientific basis for the clinical treatment.Method: Pours the uric acid sodium solution into the rat malleolus joint cavity to form the animal of acute gouty arthritis model, uses the yeast paste and the potassium oxona separately to fill the stomach into the mouse to form the hyperuricaemia model animal, and use the treatment law of removing blood stasis and dredging collateral of the acupuncture and moxibustion to treat two kind of models separately, and compares with the anti-inflammation medicine (indomethacin) and the falling uric acid medicine (benzrone and allopurinal), to observe the influence of the index of correlation of the pain, the inflammation and the blood uric acid level.Results: 1st, through the method of the mouse ear swelling as well as chemical stimulations analgesia experimental method and hot plates analgesia experimental technique the hotplate method experiment, had proven the treatment law of removing blood stasis and dredging collateral of the acupuncture and moxibustion to have the obvious effect of anti-inflammation and analgesic (p<0.05). 2nd, in the acute gouty arthritis model animal, separately determinates the level of pain mediums and the inflammatory factor of part tissues of the acute gouty arthritis rats, had proven the treatment law has the inhibitory action to them (p<0.05); Through the pathological section and the electricity mirror observation, from the pathology morphology aspect proved the success of the experimental acute gouty arthritis model and the accurate function of the treatment law. 3rd, the treatment law of removing blood stasis and dredging collateral of the acupuncture and moxibustion can obviously reduce uric acid and urea nitrogen in blood (p<0.05), has the suppression influence to the更多还原