电针淡化小鼠海洛因心理依赖的成瘾记忆机制研究

【作者】 陈洪沛；

【导师】 黄迪君；

【作者基本信息】 成都中医药大学 ， 针灸推拿学， 2006， 博士

【摘要】 目的：研究低频电针对实验性海洛因心理依赖模型的奖赏—记忆中枢生化的影响，深入揭示针灸减轻海洛因心理渴求以淡化成瘾记忆的关键机制，为针灸防治心瘾及复吸提供实验和临床依据。 方法：将经过CPP筛选的无明显天然偏性的56只雌雄昆明种小鼠随机分为空白组、模型组、电针组和西药组。按海洛因剂量逐日递增原则并进行白箱CPP训练制作海洛因心理依赖模型，以低频电针为主要治疗方法，电针海洛因心理依赖小鼠“内关”、“三阴交”及点刺“四神聪”。运用行为学测试、酶联免疫吸附和免疫组化等实验技术方法，以药物成瘾记忆形成和保持的重要靶区——海马和中脑作为切入点，研究电针对实验性海洛因心理依赖小鼠的体重、CPP值、海马的AchE和DA浓度及中脑AchE表达的影响。 结果：1．电针对实验性海洛因心理依赖小鼠模型具有促进体重恢复正常的作用。2．电针对实验性海洛因心理依赖模型具有显著降低白箱CPP值的作用。3．电针对实验性海洛因心理依赖模型具有明显升高海马Ache浓度和降低海马DA浓度的作用。4．电针可能具有促进实验性海洛因心理依赖模型中脑AchE表达降低的趋势。 结论：1．电针具有改善戒断症状、减轻心理渴求，从而淡化成瘾记忆的作用。2．电针对实验性海洛因心理依赖模型中与成瘾记忆相关的胆碱能神经系统和多巴胺系统具有良性调节作用。3．电针可能通过调控实验性海洛因心理依赖模型的奖赏—记忆中枢网络中神经递质系统之间的内稳态而发挥其治疗作用。更多还原

【Abstract】 Objective: To study the neurophysiology mechanism of addictive memory in the hippocampus and the mesencephalon on experimental heroin psychological dependence mice with the low frequency electroacupuncture more profoundly.Method: Make the experimental heroin psychological dependente model with heroin conditioned place preference (CPP) in mice, 56 mice were equally and randomly divided into 4 groups after pretesting: a contrary group, a model group, an electroacupuncture therapy group, and a model with western medicine group. needling the "Neiguan(PC6)" "Shanyinjiao(SP6)" "Sishengchong" points.The items observed includes: weight , CPP, the density of AchE and DA in hippocampus and the gray scale resolution of AchE in mesencephalon.Result: low frequency EA could significantly gain in weight, disappeared the biasing to white side of the box, reduce the density of DA and elevate the density of AchE in hippocampus on the experimental heroin psychological dependente model. Trend to express AchE in mesencephalon.Conclusion: Needling the points could suppress heroin CPP, possibly through to regulate the mechanism of neurotransmitters in the hippocampus and the mesencephalon of mice with psychological dependence on heroin by electroacupuncture (EA) therapy .to regulate the experimental heroin psychological dependente mice model in the drug rewarding- memory center formed neural network in CNS to hand over between the nature system in stable state but to play its treatment role.更多还原