【作者】 王晋；

【导师】 郭国祯； 宫越顺二；

【作者基本信息】 第四军医大学 ， 放射医学， 2006， 博士

【摘要】 电子技术的迅速发展，以及微波、射频等电子产品日趋广泛的应用，使职业人员和公众受到各种频段电磁波(electromagnetic fields，EMF)照射剂量日益增加。环境电磁场对健康的影响越来越受到人们的关注，特别是环境电磁场与肿瘤发生之间的关系更是关注的焦点。虽然EMF与癌症相关的研究已有许多，但至今尚未获得一致的结论。绝大多数肿瘤的发生表现为多阶段多步骤的复杂过程。这个过程大致可以分为三个阶段：激发阶段，促进阶段和增殖阶段。若高频电磁场(high frequency electromagnetic field，HFEMF)与癌症的发生相关则HFEMF在癌症发生过程中至少参与一个发展阶段(激发和／或促进阶段)。French等近来提出HFEMF的能量可能扮演着应激诱导物的作用，它引起蛋白构型的改变，这种改变继而引起暴露细胞热休克蛋白(heat shock proteins，HSPs)的改变，从而干扰正常细胞的调节，最终增加癌症发生的危险性。但精确的机制还不清楚。 本研究首先检测2450 MHz HFEMF对20-甲基胆蒽(20-Methylcholanthrene，MC)诱导的C3H10T1／2细胞(8克隆)细胞转化的影响；其次，以HSP70和HSP27作为应激标志研究2450 MHz HFEMFs是否更多还原

【Abstract】 In recent years, there has been a rapid increase in the use of devices and systems employing electromagnetic energy. People are concerned about the association between environmental electromagnetic fields (EMF) and cancer. The possible mechanisms of the induced effects remain unclear, although a number of theoretical models have been proposed. Tumor formation is considered to be a multi-step process including initiation (mutation), promotion and progression. Whatsoever, if EMF could contribute to the carcinogenic progress, it would affect one or more steps (initiation and/or promotion) in the neoplastic transformation process. A recent publication by French et al. suggests that RF field energy primarily acts as a stress inducer, causing changes in protein conformation, which in turn cause a synthesis of heat shock proteins (HSPs) in exposed cells and tissues. These changes may disturb the normal regulation of the cells and ultimately lead to an increased risk of cancer. However, the exact mechanisms remain unknown.First, this study was to investigate the possible effects of 2450 MHz continuous wave (CW) high frequency electromagnetic fields (HFEMFs) at different specific absorption rates (SARs) on 20-Methylcholanthrene (MC) induced transformation in C3H10T1/2 cells; second, we investigated whether更多还原