【作者】 秦明；

【导师】 饶志仁；

【作者基本信息】 第四军医大学 ， 神经生物学， 2006， 博士

【摘要】 有关疼痛机制研究一直都是神经科学研究的一个热点。内脏痛与躯体痛既有相似之处，也有不同区别点。内脏痛的性质模糊，定位不明确，机体的反应强烈，可引起出汗、心悸气短。目前有关内脏疼痛信息传递的内在机制仍不明确，以至在临床治疗上一直是个难题。 目的：观察躯体痛刺激（针刺足三里穴）干预前后，腹腔注射乙酸致内脏痛模型大鼠中枢神经系统（延髓内脏带及脊髓）中神经元和胶质细胞（主要是星形胶质细胞和小胶质细胞）在光、电镜下的形态学反应，以探讨神经元和胶质细胞在针刺镇痛和内脏痛觉信息传递中的作用及其相互关系，为临床治疗内脏痛提供理论依据。 方法：通过计算内脏痛行为学积分观察针刺足三里穴干预前后腹腔注射乙酸致内脏痛模型大鼠的行为学变化；采用免疫组织化学技术观察延髓内脏带及脊髓后角中即可早期基因Fos、胶质原纤维酸性蛋白（Glial Fibrillary Acidic Protein,GFAP）和OX42的表达变化、分布和相互关系；采用免疫组织化学方法，观察椎管鞘内注射P2X₄、P2X₇受体激动剂和拮抗剂干预前后内更多还原

【Abstract】 The study of pain mechanism is always the interested point in neuroscience research. There is similar and different characteristic between visceral and somatic pain. In visceral pain, its quality is obscure, its accurate position in body is unclear, the response of body is greatly intense, such as sudation, tachycardia, breathe hard et al. Effective therapy for visceral pain is lacking, and the underlying mechanisms about the visceral noxious signal transmitting in the brain are poorly understood.Objective To study the function and relationship of neurons and glial cells in visceral noxious signal transmission and electroacupuncture analgesic effects in visceral pain(VP) model rats induced by intraperitoneal(i.p.) injection of 0.6% acetic acid(AA), by using single or double immunohistochemical methods and immunoelectron microscopy, we investigated the morphological response and relationship of neurons and glial cells in medullary visceral zone(MVZ) and spinal cord.更多还原