【作者】 吴玉杰；

【导师】 贾连顺； 袁文； 吴海山； 肖建如； 陈雄生；

【作者基本信息】 第二军医大学 ， 外科学， 2006， 博士

【摘要】 背景 急性脊髓损伤(spinal cord injury，SCI)是指脊髓遭受到外力打击后导致完全性或不完全性脊髓运动、感觉、括约肌功能障碍等，是脊柱外科领域中一种常见且破坏性极大的疾病。随着脊髓损伤基础及临床研究的不断深入，脊髓损伤的治疗也得到不断发展。近年，不同学者采用不同方法治疗脊髓损伤病人，并取得一定的效果，也有报道一些药物有助于脊髓损伤后功能恢复。然而所有结果仍不尽人意。为寻求一种新的治疗脊髓损伤的方法，根据脊髓损伤后病理改变，最大限度减少这种病理改变将是一种有效治疗方法。全身性应用药物通过血循环途径达到脊髓损伤部位，可能会有效的打断脊髓损伤后病理进程从而达到治疗脊髓损伤、保护或者恢复脊髓功能的目的。为此，进一步研究一种较好的用药品种及用药程式将是一种非常有前景的方法。 目的 1．探讨应用药物治疗脊髓损伤行为学的改变； 2．探讨应用药物治疗脊髓损伤病理学的改变； 3．探讨应用药物治疗脊髓损伤神经细丝及胶质纤维酸性蛋白表达的影响； 4．探讨应用药物治疗脊髓损伤对细胞凋亡的影响。 5．通过结果探讨不同药物对大鼠急性脊髓损伤的治疗作用，为临床上应用药物治疗脊髓损伤提供理论指导。 方法 实验中将216只大鼠分为对照组及6种不同药物组。采用腹腔内药物注射进行麻醉后，利用改良Allell’s打击法建立大鼠急性脊髓损伤模型。手术后通过尾静脉给予药物。在术后8h、24h、4天、7天、14天分别进行以下指标检测：1、采用胥少汀对脊髓损伤的四肢行走动物提出的后肢6级行为学评分障碍率及更多还原

【Abstract】 BACKGROUNDAcute spinal cord injury(SCI) is defined as an insult to the spinal cord that partially or completely interrupts the three main functions of the cord-motor, sensory, and reflex activities. It is mostly caused by motor vehicle accidents, falls, sports injuries and some other wounds. During years of unremitting efforts by scientists of many countries, with basic and clinical research of the spinal cord injury, treatment to the spinal cord injury has been developed much. However, it is not satisfactory. Considering the process of SCI, to decrase or to block the process may be an effective method. Though some drugs showed much effect in proctecting and improving the spinal function after injury, searching for an better means to improve its function is still an important subject to be solved.OBJECTIVETo study the effect of different drugs to the behavior and neurological changes , pathological and ultrastructural changes , the expression of neurofilament (NF) and glial fibrillary acidic protein (GFAP) ,and nerve cell apoptosis of spinal cord in rats after spinal cord injury. Then to find an effective method to cure SCI.METHODSTwo hundreds and sixteen healthy adult Sprague-Dawley rats were randomly divided into seven groups:blank control group, control group, experimental group. Build acute spinal cord injury model in rats with modified Allen’s method after rats were anesthesed with drug administration through abdomin. Different drugs were given to cure spinal cord injury through tail vein each day. The follow index were observed at 8h、24h、96h、7day、14day:(1) to evaluate the function of spinal cord by更多还原