【作者】 李崖雪；

【导师】 孙申田；

【作者基本信息】 黑龙江中医药大学 ， 针灸推拿学， 2006， 博士

【摘要】 目的:研究电针大鼠下关穴、地仓穴治疗三叉神经痛的有效性及其镇痛机理。 方法:1、脑闩下蛛网膜下腔注射微量青霉素G-K制造大白鼠三叉神经痛样反应模型。2、观察三叉神经痛反应模型成立后,对照组、卡马西平组、电针组总发作次数,自发反应持续时间,诱发反应阳性时间。3、用放射免疫法测定电针组、卡马西平组、对照组、假手术组血浆P物质(sp),β—内啡肽(β—EP)的含量。4、用肌电图仪测定对照组、卡马西平组、电针组,因刺激大白鼠眶下神经所诱发的咬肌肌电反应的潜伏期及基础痛阈(引起咬肌诱发电位的最小刺激量)。 结果: 1、电针组、卡马西平组与对照组相比可明显减少大鼠三叉神经痛样反应的总发作次数,缩短自发反应持续时间及诱发反应阳性时间(P<0.01);卡马西平组与电针组比较,各项指标无显著性差异(P>0.05)。 2、电针组P物质含量明显低于对照组(P<0.01)电针组与卡马西平组比较无显著性差异(P>0.05)。电针组β—内啡肽含量明显高于假手术组、对照组(P<0.01)电针组与卡马西平组比较无显著性差异(P>0.05)。 3、与对照组相比,电针后即时由眶下神经刺激诱发的咬肌肌电反应的潜伏期明显延长(P<0.01),30min左右达到高峰(P<0.01),持续约30分钟,然后逐渐下降,90min明显下降。电针组于电针后30min潜伏期较卡马西平组明显延长(P<0.05)与对照组比较卡马西平灌胃后,即时潜伏期明显延长(P<0.05),30min、60min、90min、120min潜伏期明显延长(P<0.01),无明显峰值。更多还原

【Abstract】 Acupuncture therapy, accepted widely as " green therapy " which is safe,eutherapeutic, economic and convenient,has been largely proved effective on trigeminal neuralgia.This experiment is divided into three parts. The first part is that thirty wistar rats are divided into three groups at random. The first group is negative group(without any treatment).The second group is carbamazepine treatment group.The third group is electroacupuncture treatment group. From observing the attach number of TN of the model animals, the duration of the spontaneous reaction and the positive time of the induced response draw a conclusin.The result show that electroacupuncture differed significantly from the negative group in number(P<0.01) but slightly different from that of carbamazepine (P>0.05).The sequential trial has indentified that electroacupuncture could reduce the attack number of TN of the model animal,shorten the duration of spontaneous reaction, lighten the degree of the attack TN of the model animals. Electroacupuncture is a effective treatment in TN.The second part is that forty wistar rats are divided into four groups at random.The first group is sham operation group(trigeminal nerve not injured).The second group is negative group(without any treatment).The third group is carbamazepine treatment group.The fourth group is electroacupuncture treatment group. Establish theanimal model with trigeminal neuralgia in rat. Measure the concentration of substance P and |3 -EP of blood by radioimmunological analysis.Result:The concentration of SP in electroacupuncture treatment group is significantly lower than that in negative group(P<0. 01). The concentration of SP in electroacupuncture treatment group and carbamazepine treatment group has no significant difference(P>0. 05). The concentration of ￡ -EP in electroacupuncture treatment group is significantly higher than that in sham operation group and negative group (P<0.01).The ￡ -EP in electroacupuncture treatment group and carbamazepine treatment group in not sigficantly different (P>0.05).Conclusions:Electroacupuncture may reduce the content of substance P mediating the nociceptive signals and increase the content of |3 -EP having analgesic opioid reactive substance. In the end,from this way relieve trigeminal neuralgia.The three part is that thirty wistar rats are divided into three groups.The first group is negative group;the second group is carbamazepine treatment group;the third group is electroacupuncture treatment group.The recording electrode is put in the right masseter muscles and the effect of electroacupuncture on basic pain threshold of the reflex and its latency are observed. Results:The latency lengthened and basic pain threshold increased begin with after electroacuputure, reach peak at about 30min,remain high level from 30~60min and begin falling at 60min. The wave has not been in negative group and carbamazepine treatment group but in latency and basic pain threshold there are significantly different betweenthem (P<0.01).At 30min contrast with the carbamazepine treatment group,the latency of the electroacupuncture treatment group is longer and the basic pain threshold of it is higher (P<0.05). Conclusions: Electroacupuncture have obvious effect on relieving the trigeminal neuralgia.更多还原