【作者】 廖龙标；

【导师】 肖贤明；

【作者基本信息】 中国科学院研究生院（广州地球化学研究所） ， 环境科学， 2006， 博士

【摘要】 水体被污染主要有化学性,物理性和生物性三大类。目前,国内有关水环境生物性污染的报道较少。珠江在全国七大流域中是水质较好的流域之一,但局部水环境污染依然相当严重,主要集中在珠江三角洲地区。本研究以珠江广州河段的生物学污染为主要研究对象,综合运用生物学,化学,环境科学,电化学和分子生物学等多门学科的有关理论,研究方法和检测手段,对水体生物污染的检测和修复进行了初步探讨和研究,主要取得了以下几方面的成果或认识:运用固相合成法合成二茂铁肽-DNA的共轭化合物;制备出以二茂铁肽为基础的电化学生物传感器,并对其形貌和非特征性吸收做了研究;实现了运用二茂铁肽电化学生物传感器来检测水环境中的有害细菌或病毒;研究了制备酸性电解水时进水速度,食盐浓度和温度等不同实验条件对氧化还原电位的影响;也研究了酸性电解水在半密封和半密封保存时pH值、氧化还原电位、有效氯、电导率和溶解氧等性质的变化情况,在此基础上揭示了酸性电解水形成高氧化还原电位的原因;以大肠杆菌为研究对象研究了酸性电解水的高氧化还原电位灭菌的机理;为水环境的生物污染修复做了初步模拟性探讨,发现生物性污染的水样通过电解可以灭活细菌或病毒,并且,不会影响水体其他理化性质。 本研究成果不仅实现了运用现代分子诊断技术方便而有效地检测水环境的生物污染,而且对水环境的生物污染修复有一定的指导意义。相对于常规的检测手段和修复方法,本研究都进行了新的尝试。更多还原

【Abstract】 Water bodies are primarily polluted from chemical issues, physical issues and microbiological issues. At present, the research of water pollution of biology is seldom reported. The Pearl River is one of the best rivers over the 7 great rivers in China. However, parts of the Pearl River are seriously contaminated, especially in the region of the Pearl River Delta. The objective is the region of Guangzhou in the Pearl River here, using the relative theories, methods and techniques from biology, chemistry, environmental sciences, electrochemistry and molecular biology etc., the biological contamination of Guangzhou parts of the Pearl River is primarily discussed and studied involved in its detection and rehabilitation. The primary research achievements and discoveries are showed below: the synthesis of ferrocenyl peptide-DNA conjugate using solid phase peptide synthesis;the preparation of ferrocenyl peptide based electrochemical biological biosensor, additionally, its morphology and non-specific adsorption are also studied;ferrocenyl peptide electrochemical biosensor is successively applied in the detection of toxic bacterial and viruses;the effects of inlet water rate, concentration of sodium chloride and temperature on the oxidation-reduction potential are also studied;the changes of characters such as the value of pH, oxidation reduction potential, free chlorine, conductivity, dissolved oxygen etc. at the semi-closed and closed states are studied, based on those, the reveal of the reason that electrolyzed oxidation water can have high oxidation reduction potentials;the new mechanism of disinfection for oxidation reduction potential using electrolyzed oxidation water based on the objective of E. coli;the primary and simulated research for the rehabilitation of biological contamination in an aqueous environment, and the bacterial or viruses in the biological pollution water sample are inactivated by means of electrolysis, in addition, which have not a effect on other physical-chemical characters of the water sample.The primary research achievements show that the convenient and effective detection of biological contamination in an aqueous environment using modern molecularly diagnosed techniques and the direction to the rehabilitation of biological contamination in an aqueous environment. Comparison with conventional detection and rehabilitation techniques and methods, the novel studies are performed in this project.更多还原