【作者】 贾秀川；

【导师】 刘怀军；

【作者基本信息】 河北医科大学 ， 神经病学， 2006， 博士

【摘要】 第一部分线栓法兔局灶性脑缺血/再灌注模型的制作及影像学评价目的:探讨建立稳定的线栓法兔脑缺血/再灌注模型,并对其进行MR影像学和神经功能评价。方法:健康新西兰白兔40只,随机分为三组:A组5只,进行颈内动脉血管造影检查;B组5只,为假手术组;C组30只,选择直径0.45mm的导丝直接从CCA经ICA插至MCA起始部,3h后抽回导丝至CCA内进行线栓法局灶性脑缺血/再灌注模型的制作,并利用MRI和神经功能缺损评分对模型进行评价。结果:血管造影可以清晰地显示颈内动脉的走行及其颅内分支情况,22只动物脑缺血/再灌注模型成功。MRI能清晰显示脑组织的病理改变,脑缺血/再灌注后1h神经功能缺损评分为2.8±0.45。结论:采用直径0.45mm的导丝直接从CCA经ICA插至MCA起始部,再灌注时,抽回导丝至CCA内可用于建立较稳定(22/30)、长期存活并适合影像学研究的脑缺血/再灌注模型。第二部分兔局灶性脑缺血再灌注损伤后的MRI表现和病理改变对照研究目的:研究兔局灶性脑缺血/再灌注损伤后的系列MRI动态演变过程,并将其与病理改变相对照。方法:健康新西兰白兔27只,随机分为二组:A组5只,为假手术组;B组22只,为线栓法局灶性脑缺血/再灌注模型组。于缺血3h/再灌注后1h(4只)、3h、6h、12h、1d、3d、7d(各3只)行头颅MR扫描,扫描序列包括T1WI、T2WI、FLAIR(液体衰减反转恢复)、DWI(弥散加权成像)、PWI(灌注加权成像)及强化后T1WI扫描,观察缺血再灌注后脑组织T2WI、DWI、PWI、强化后T1WI信号参数以及梗死体积的改变。兔脑切片光镜HE染色及电镜下观察缺血区组织形态学变化,同时应用免疫组化法观察缺血区GFAP、MAP-2、HSP-70及MMP-9的演变过程。更多还原

【Abstract】 Part I: The setting up of intraluminal thread approach in making focal cerebral ischemia reperfusion model in rabbit and evaluation of image featuresObjective: To establish a stable model of intraluminal thread approach in making focal cerebral ischemia reperfusion model in rabbit and to evaluate it with magnetic resonance imaging (MRI) and neurological score.Methods: Forty white New Zealand rabbits were randomly divided into three groups:A,B,and C.Angiography of internal carotid artery(ICA) was performed in Group A(n=5). Control Group B(n=5) was sham-operated.In Group C(n=30),the focal cerebral ischemia reperfusion model was made by inserting a wire(d=0.45mm) into middle cerebral artery(MCA) through ICA and common carotid artery(CCA) directly and pulling it out 3 hours later. MRI and neurological deficit score were used to evaluate the effectiveness of models.Results: Angiography can show the course and bifurcation of ICA clearly.22 models were successful.The pathological changes were shown on MRI.The neurological deficit score (reperfusion 1h) was 2.8±0.45.Conclusion: The focal cerebral ischemia reperfusion model made by inserting a wire(d=0.45mm) into MCA through ICA and CCA directly and pulling it out 3 hours later was stable and fit for imaging research.Part II: The serial MRI after rabbit focal cerebral ischemia reperfusion injury and its correlation with pathological changesObjective: To explore the serial MRI after rabbit focal cerebral ischemia reperfusion injury and correlate it with the pathological changes.Methods: 27 white New Zealand rabbits were randomly divided into two groups:A and B.Control Group A(n=5) was sham-operated.In Group更多还原