【作者】 范哲；

【导师】 李广生；

【作者基本信息】 吉林大学 ， 病理学与病理生理学， 2006， 博士

【摘要】 本实验以氟中毒大鼠动物模型和体外成骨细胞染氟培养为研究对象,应用形态学、免疫组织(细胞)化学、原位杂交、Western blot和ELISA等技术,着重对氟化物作用时OCN、OPN、FN、ON及与其密切相关的TGF-β从mRNA到蛋白质的表达进行了探讨。结果表明,在氟中毒大鼠骨组织OCN、OPN、FN、ON等四种非胶原蛋白和TGF-β的表达在投氟组与对照组之间未发现明显差别。在体外培养成骨细胞,染氟1,2,4mg/L,培养48,72h,可在mRNA到蛋白质水平显著促进这四种非胶原蛋白和TGF-β的表达。氟作用下这些非胶原蛋白的合成和分泌增多,很可能在进一步促进成骨细胞增殖、活化和调节矿化等方面发挥作用。本研究为全面了解氟骨症的发生机制提供了有价值的新资料。更多还原

【Abstract】 The bone damage of chronological fluorosis relates to multi-cells and extracellular matrix, which participate in bone transition.Non-collagenous proteins,as the important ingredient of extracellular matrix,play an important role in the osteogenesis and bone transition.But the systematical and further research on the influence of related fluorides to non-collagenous bone matrix proteins and the function of proteins of transformation of bone matrix to the development of fluorosis of bone,were rarely done.Via fluoride concentration in drinking water to replicate casts of white-rat fluorosis and culture in vitro osteoblast fluoridized,and adopting the technology of morphology, immunohistochemistry,in situ hybridization,RT-PCR,Western blot and ELISA,this experiment mainly carried out the research on the influence of fluoride to the following non-collagenous proteins,such as osteocalcin,osteopontin,osteonetin,as well as the expression of transforming growth factorβwhich closely relates to them.By observing the bone tissue casts of fluorotic rats, bony trobecula of metaphysis crowded together;osteoblast and osteoclast increase tremendously,and更多还原