刚地弓浆虫无性生殖及弓浆虫病病理学研究

【作者】 汪琦；

【导师】 林宇光；

【作者基本信息】 厦门大学 ， 动物学， 2000， 博士

【摘要】 作者采用涂片、染色的经典生物学方法,结合SDS-PAGE技术、RAPD技术、RT-PCR技术对RH株弓浆虫在鼠体内无性生殖的过程进行了考察,从个体水平、蛋白质水平、DNA水平、RNA水平对虫体无性生殖过程中一系列生物学变化进行了描述,同时采用石蜡切片技术对鼠弓浆虫病的病理学作了研究,以期为弓浆虫病的防治提供科学的依据,现将研究结果报告如下: 1、不同的感染方式对鼠活力有不同的影响。弓浆虫RH株速殖子对宿主鼠的急性感染,导致鼠的死亡,但鼠的存活期随感染方式的不同而存在差异,腹腔感染的小鼠死亡时间最早,口腔感染的小鼠次之,S.D.治疗的小鼠存活期明显延长,但不能避免宿主的死亡,第1代液氮冻存复苏的虫体感染力弱,鼠存活期较长,子1代虫体感染力恢复,腹腔感染后鼠在第4天死亡。 2、不同的宿主细胞,虫体在其中增殖的能力不同,以来源于小鼠的巨噬细胞、脾细胞、肾细胞作为宿主细胞,虫体在巨噬细胞中的增殖能力最强,在脾细胞中的增殖能力次之,在肾细胞中的增殖能力最弱;不同发育阶段的虫体,感染力不同,实验结果表明,处于增殖期的虫体,其感染力低,而处于发育成熟期的虫体,感染力强,成熟后突破宿主细胞膜后散出的虫体,感染力最强;处于同一增殖发育阶段的虫体,对不同宿主细胞的感染力也不相同,对鼠巨噬细胞的感染力最大,脾细胞次之,肾细胞的感染力最低;小牛血清的有无对虫体的侵袭力和增殖都有一定的影响,10%的小牛血清可增加虫体的侵袭力,也可增加虫体在宿主细胞内的增殖,未加入小牛血清时,未纯化的速殖子在体外宿主细胞内仍可增殖,但增殖力明显不如加小牛血清组。 3、弓浆虫无性生殖的方式有二裂殖、出芽生殖、裂殖生殖等方式,其中裂殖生殖是最常见的生殖方式,巨噬细胞、肝细胞、脾细胞及心肌细胞中的虫体都可进行裂殖生殖,细胞内和细胞外的虫体也都可进行裂殖生殖。弓浆虫无性生殖时常形成群落,作者所观察到的群落有环型、半环型、玫瑰花更多还原

【Abstract】 The asexual proliferation of Toxoplasma gondii (RH strain ,international standard strain)in mice were studied with stain technique,SDS-PAGE technique,RAPD technique and RT-PCR technique.The changes in individual level ,protein level,DNA level and RNA level were elabrated in this article.At the same time the pathology of mice toxoplasmosis was studied too.The result showed as follows:1 .The various infection ways affected the life of the mices.The tachyzoites of Toxoplasma gondii (RH strain) always led to the death of the mices.But the ways of infection led to the differences of the mice life .The infected mice with peritoneumly injection died firstly,the mice infectd with mouth innocation died secondly and S.D. could prolong the days of mice life ,but couldn’t avoid the death.The infection ability of tachyzoites preserved by liquid Nitric was decreased..2.The proliferation ability of toxoplasma gondii changed with the host cells.It was most strong in M φ and weakest in kedney cells.The infection ability changed with the various maturing stages of Toxoplasma gondii.The tachyzoites in mature stage had the most strong infection ability, could increase the infection ability and proliferation ability of Toxoplasma gondii.3.The production ways of Toxoplasma gondii included binary fission,budding, schizogony.Among these ways schizogony was the most common way and it could be conducted in Mφ s, liver cells,spleen cells and heart cells.Clones were always formed during the asexual production and five pattern clones were oberserved .The characteristic of clone’s distributionrelated to the host cells. 4.The tachyzoites could avoid the immune inhibition and proliferated in M φ .The tachyzoites developted after infection and mutured at 72h after infection in the pseudocysts.Matured and free tachyzoites moved actively .更多还原