【作者】 刘拥军；

【导师】 段富津；

【作者基本信息】 黑龙江中医药大学 ， 方剂学， 2005， 博士

【摘要】 银屑病是一种常见并易复发的慢性炎症性疾病,本病病程较长,病因及发病机制未明,可能与遗传、感染、代谢、内分泌、神经精神、免疫功能障碍有关。中医药治疗银屑病疗效可靠,副作用小,成为现在研究的趋势。 银屑病是多基因遗传背景下T细胞失常的免疫性疾病。在临床上银消丸用于热毒血瘀型银屑病的治疗。本研究的目的是观察中药银消丸对银屑病小鼠模型的治疗作用及免疫功能的影响。用鼠尾鳞片表皮模型、雌激素期阴道上皮模型及增殖细胞核抗原(PCNA)免疫组化方法观察,检测ConA诱导的T淋巴细胞增殖,分泌细胞因子IFN-γ,IL-2,IL-6的水平以及增殖细胞核抗原表达情况。实验结果显示银消丸可促进鼠尾鳞片颗粒层的形成,在电镜下可见多层颗粒细胞,细胞较大;对小鼠阴道上皮细胞有丝分裂有抑制作用,在电镜下可见细胞核与细胞器无异常改变。银消丸还可抑制ConA诱导的T淋巴细胞增殖及分泌细胞因子IFN-γ,IL-2,IL-6的水平,抑制增殖细胞核抗原表达。由此推断,银消丸可能通过抑制表皮细胞增殖与促进其分化,抑制增殖细胞核抗原表达,参与抗炎与免疫调节过程来发挥治疗作用的。银消丸对银屑病小鼠模型有显著的治疗作用,是多靶位治疗银屑病的有效药物,本研究为中医药治疗银屑病提供了理论依据。更多还原

【Abstract】 Psoriasis is a commonly and frequently encountered chronic inflammatory dermal disease. The duration of this disease is comparatively longer ,the etiology and pathogenesis of this disease is still unknown ,it may be related to genetic ,infective, and metabolic, endocrine, nervous, mental, and immunological disturbance. In traditional Chinese medicine (TCM)this disease is called "Bai Pei". TCM gives full play to cure psoriasis ,whose side effect is small , curative effect is reliable , and is the trend of our research now.Psoriasis is a immune disease of the T cell disorder which is due to poly gene genetics. Yin Xiao Bolus were used for syndrome of stagnation and obstruction of heat toxin and blood stasis in clinic. This study aimed to observe the effect of Yin Xiao Bolus on psoriatic models and immune function in mice. Yin Xiao Bolus were studied by using the modle of tail scales of mouse and vaginal epithelium, using the mouse experimental model of the technique of PCNA immunohistohemistry, ConA-induced proliferation and production of IFN- Y ,IL-2,IL-6 by T lymphocytes.The result showed that Yin Xiao Bolus could facilitates development of granular cell layers in mice tail scales, there were more granular cell layers and bigger granular cell in scales under electronic microscope. Yin Xiao Bolus could significantly inhibit the mitosis of vaginal epithelial cells in mice treated with estrogen, No abnormal changes of nucleolus and organelle were found under electronic microscope, inhibit ConA-induced proliferation and production of IFN- γ,IL-2,IL-6 by T lymphocytes. These facts indicate that Yin Xiao Bolus are related promoting the formation of granular cells, and inhibiting hyperplasia of epidermal cells and the更多还原