【作者】 隋正红；

【导师】 张学成；

【作者基本信息】 中国海洋大学 ， 海洋生物学， 2001， 博士

【摘要】 本文利用一系列龙须菜品系,包括不同产地及不同色素突变体,用PCR 扩增的方法,得到它们的藻红蛋白亚基基因的部分序列,进行序列分析、比较。通过对色素突变体的研究,结合已有的生化结果,阐明光合作用蛋白性质变异的分子机理,结果显示基因变异导致藻红蛋白的空间构型的改变,并可能影响藻红蛋白的光谱特性;对不同产地藻株藻红蛋白序列的分析表明藻红蛋白亚基基因序列的保守性,并用该基因序列进行了系统研究,所得结果与传统分类结果基本一致,藻红蛋白基因序列可用于不同种间及以上水平亲源关系的分析,而不能用于种群间亲源关系分析;在国内外首次报道了龙须菜藻红蛋白β、α亚基基因全序列,并在原核体系大肠杆菌中进行了表达,得到了17KD 的表达产物;在委内瑞拉野生型中扩增到一个可编码185 个氨基酸的开放读码框架。本结果对进一步藻红蛋白基因结构的研究打下基础。更多还原

【Abstract】 A series of Gracilaria lemaneiformis from different habitates and different pigment mutants were employed in the present studies. The partial gene sequences of their phycoerythrin subunits were obtained by using PCR amplyfication, and further compared and analyzed. The studies aimed to disclose the molecular mechanism of the change of photosynthetic proteins by combining known biochemical results to study on the pigment mutants. The results showed that gene deviations led to the spatial structure change of phycoerythrin, which would effect on the spectral character of this protein. The gene sequence was highly conserved among different strains. The sequence was applied on the phylogenetic studies. The result was in accord with that of the traditional one. Phycoerythrin gene sequence was suggested to be useful in delineating relationship within species and higher than that, but was unfitted in the studies within populations. The studies reported the whole sequence of alfa and beta genes of phycoerythrin subunits of Gracilaria lemaneiformis for the first time. It was then expressed in E.coli. A product with a molecule weigh of 17KD was obtained. A fragment coding for an open reading frame with deduced 185 amino acids was amplified. The results will enhance the studies of phycoerythrin gene structure.更多还原