【作者】 刘英；

【导师】 周翠英；

【作者基本信息】 山东中医药大学 ， 中西医结合临床， 2005， 博士

【摘要】 目的:建立干燥综合征（Sj（?）gren’s syndrome SS）动物模型,观察清燥布津法对SS小鼠模型下颌腺aquaporin-5（AQP₅）表达的影响。方法:雌性BALB/c小鼠颌下腺匀浆离心后的上清液加弗氏完全佐剂（CFA）制成乳化免疫抗原,采用多点注射法免疫同系鼠,同时注射白百破疫苗建立SS小鼠模型。以环戊硫酮为对照组,观察中药对SS小鼠模型唾液分泌、病理及下颌腺AQP₅（免疫组化法）表达的影响。结果:模型组与正常对照组比较,小鼠的体重、唾液流率下降,饮水量增加。下颌腺组织中淋巴细胞重度浸润。腺体破坏。免疫组化方法显示:AQP₅在腺体的分布和表达减少或消失。中药高剂量组与模型组比较,小鼠的体重、唾液流率增加,饮水量减少。下颌腺组织中只有淋巴细胞的轻度浸润。腺体的结构正常,无破坏。AQP₅在腺泡、闰管、导管等处均有较强表达。中药高剂量组疗效优于其他治疗组。结论:清燥布津法有明显抑制腺体破坏,改善唾液分泌功能的作用,其作用机理可能与抑制免疫炎症,促进AQP₅在小鼠下颌腺的表达有关。更多还原

【Abstract】 Objective: To establish a experimental SjOgren’s syndrome (SS) model and observe the effect of QZBJ therapeutic method on expression of aquaporin-5 (AQP5) in SS mice. Methods: BALB/c mice were injected with homologous antigen of submandibular gland tissues plus complete Freund’s adjuvant ( CFA ) plus diptheria-tetanus toxoids-pertussis to enhance the immunological reaction. The same immunological way repeated twice to duplicate SS model. Treating with Anethol Trithione as control group, saliva flow rate test, histopathological findings and AQP5 expression in submandibular glands were observed. Results: Obvious losing of weight and saliva, increasing weight of drinking water were found in SS models, and there were also massive lymphocytic infiltration and reduced number of the acinar in submandibular gland of BALB/c nice, where AQP5 expression decreased or disappeared. High-dosage QZBJ Mixtrue could obviously increase weight and excretion of salivary glands while decrease drinking water. AQP5 was strongly expressed in the acinar and the secretory ducts in high-dose QZBJ Mixtrue group. Conclusion: QZBJ therapeutic method obviously inhibits lesions of salivary glands and improves functions of salivary glands. QZBJ therapeutic method might treat SS by inhibiting immunlogical inflammation and affecting expression of AQP5 in salivary glands.更多还原