【作者】 聂江力；

【导师】 祖元刚；

【作者基本信息】 东北林业大学 ， 植物学， 2003， 博士

【摘要】 本文通过宏观与微观相结合的手段，确定了五味子木脂素成分的超临界二氧化碳萃取的最佳工艺，对北五味子的不同产地的质量做出了综合性、系统性的评价。 1 全面系统地确定了测定五味子总木脂素含量的紫外分光光度法和五味子甲素、五味子乙素含量的高效液相分析方法。 2 对影响超临界二氧化碳萃取五味子木脂素的传质过程和溶解度因素进行了研究，确定了最佳工艺参数。实验结果表明超临界二氧化碳萃取五味子总木脂素的萃取率为1.015％，是索氏提取法五味子总木脂素的提取率的1.5倍。 3 通过不同产地北五味子HPLC指纹图谱分析与比较，表明四个产地北五味子在化合物组成方面具有明显相似性；通过HPLC指纹图谱相似度计算，表明苇河产北五味子与桃山产地北五味子相似度较高。通过对不同产地北五味子总木脂素、五味子甲素、五味子乙素的含量比较，表明苇河产北五味子木脂素成分含量值较高。 4 通过人工神经网络模拟技术对不同产地北五味子的宏观结实量进行模拟分析，表明桃山地区与苇河地区较适宜生长北五味子，其单位面积内宏观结实量较高。 通过对五味子有效成分的超临界二氧化碳萃取工艺的研究，实现了五味子绿色提取的目标；通过对不同产地北五味子HPLC指纹图谱比较与分析，以及对不同产地北五味子宏观结实量人工神经网络模拟技术的模拟分析，为评价五味子产地的质量提供可靠的科学依据及方法学的研究，从而为医药市场提供优良的五味子生药，为中药规范性实施以及提高中药标准化程度提供了必要的保证。为进一步开发利用五味子提供新的途径。更多还原

【Abstract】 The best process had been built by using supercritical carbon dioxide(CO2)to extracting lignans from Schisandra chinensis, and different originalqualities of S.chinensis were evaluated systematically and comprehensively.Both macroscopical and microcosmical methods had been utilized anddeveloped in this paper.1 The ultraviolet and visible spectrophotometry(UV-vis) for measuring the whole lignans contents and the high performance liquid chromatography(HPLC) for measuring deoxyschisandrin and Y -schisandrin contents were generally and systematically determined.2 The transferring substance curse and solubility factors that affected Lignans extraction by using supercritical CO2 were studied, and the best process parameter were determined. The experiment result showed that the extraction rate of lignans from S.chinensis by using supercritical CO2 was 1.015%, which was 1.5 times of traditional method.3 By analyzing and comparing the high performance liquid chromatography fingerprint spectrum(HPLC-FPS) of different S. cinensis origins, the compound composition showed obviously comparability in four S.chinensis origins; by computing from HPLC-FPS the similarity degree of S.chinensis was higher between Weihe and Taoshan origins than any others. By comparing lignans, deoxyschisandrin and y -schisandrin in different S. chinensis origins, the lignans compound contents were much higher in Weihe than other origins.4 Modelling fruit set in different S. chinensis origins by the artificial neural network technology, the result showed Taoshan and Weihe territories were more suitable for S. chinensis growing.By studying supercritical CO2 for extracting lignans from S. chinensis, the green extraction aim was realized; by comparing and analyzing HPLC-FPS in different S. chinensis origins and imitating and analyzing fruit set of S. chinensis from artificial neural network technology, the reliable basis and research method on evaluating quality of different S. chinensis origins were provided; preferable S. chinensis for medical market could be provided; the essential guarantee for criterion of Traditional Chinese Medicine was likely implemented and standardization was improved. A new way to exploit S. chinensis was provided further.更多还原