电针对老年性痴呆大鼠海马组织信号转导介质的调节作用研究

【作者】 刘雨星；

【导师】 梁繁荣；

【作者基本信息】 成都中医药大学 ， 针灸推拿学， 2003， 博士

【摘要】 目的：探讨电针对老年性痴呆海马等组织信号转导紊乱的调节作用，深化电针治疗AD的作用机理研究。 方法：以老年SD大鼠为研究对象，采用穹隆—海马伞机械损害的造模方法；依据“补肾活血，醒脑开窍”的原则电针刺激“百会”、“涌泉”、“太溪”、“血海”。观察了电针治疗对老年性痴呆大鼠的学习记忆行为的影响，并应用放免和免疫组化法观察了电针对中枢海马组织中信号转导介质如PKC、PTK、cGMP、nNOS，以及中隔区ChAT等指标的调节作用。 结果：穹隆-海马伞损伤后大鼠海马组织中PKC、PTK、cGMP、nNOS和中隔区ChAT的活性均显著降低，同时伴有学习记忆障碍。电针治疗可提高AD大鼠海马组织中nNOS和中隔区ChAT的活性，有效激活海马组织胞膜PKC与PTK的活性，提高AD大鼠的学习记忆能力，达到有效治疗AD。 结论：电针能有效改善AD大鼠的学习记忆障碍，其可能机制是电针能激活中隔区ChAT，海马组织中nNOS、PKC等的活性。因此，电针是临床可推广应用治疗AD的有效手段。更多还原

【Abstract】 Objective: To study the regulating functions on the signal conduction transmitters in the hippocumpus area of Alzheimer’s disease by electro-acupuncture, and develop the mechnism of electro-acupuncture on it.Methods: The senile SD rats were taken as the researching subjects, and AD model was built by cutting off the fimbria of the hippocampus. Acupoints like "Baihui"(Du20) , " Yongquan"(KI1) , " Xuehai"(SP10) ,"Taixi"(KI3) were electro-punctured according to the therapeutic princile of "Tonifying Kidney and promoting blood circulation, Reviving brain and inducing resuscitation". The effects on improving the study and memory ability of AD model rats was observed, and the regulating effects on signal conduction transmitters in hippocampus, were detected by using radio-immunoassay and immuno-histo-chemical method, which treated by electro-acupuncture.Results: The activities of ChAT in septum, PKC\PTK\cGMP\nNOS in hippocampus of the rats were reduced obviously after cutting off the fimbria of the hippocampus, meanwhile accompanied with the disorders of learning and memory. Electro-acupuncture could improve the activities of nNOS in hippocampus and ChAT in septum, encourage the activities of PKV\PTK of membrane in hippocampus tissues effectively, and promote the learning and memory abilities of AD model rats.Conclusion: Electro-acupuncture could improve the learning and memory of AD rats, and its mechanism may be that acupuncture could encourage the activities of nNOS\PKC in hippocampus, and that of ChAT in septum. Thus, electroacupuncture could be applied for AD as an effective treatment.更多还原