【作者】 赵岩；

【导师】 马宝璋；

【作者基本信息】 黑龙江中医药大学 ， 中医妇科学， 2003， 博士

【摘要】 目的： 探讨子宫内膜异位症的发病机理和异位宁治疗子宫内膜异位症的作用机制。 方法： 在成功建立大鼠子宫内膜异位症模型的基础上，我们观察了形态学，并分别采用酶联免疫吸附法、免疫组化、原位杂交方法检测了血清IL-2、IL-10水平，局部病灶组织EGF、PDGF、bcl-2、bax蛋白阳性表达及bcl-2mRNA、baxmRNA的阳性表达。 结果： 1、子宫内膜异位大鼠血清IL-2水平较正常组低，而IL-10水平较正常组高。 2、子宫内膜异位大鼠局部病灶组织，bcl-2mRNA及蛋白的阳性表达较正常组高，而baxmRNA及蛋白的阳性表达较正常组低。 3、子宫内膜异位大鼠局部病灶组织EGF和PDGF蛋白的阳性表达较正常组高。 结论： 1、免疫调节失衡与子宫内膜异位症的发生发展有关。 2、细胞凋亡能力降低在子宫内膜异位症的发展中起重要作用。 3、血管生成促进了异位内膜的种植和生长。 4、异位宁能够调整免疫失衡，诱导异位内膜细胞凋亡，抑制异位内膜组织新生血管生成。更多还原

【Abstract】 Purpose:To explore the pathogensis of endometriosis and therapeutic mechanism ofYWN.Method:On successfully creating EM model of rats, we investigate histomorphology, the level of interleukin-2 (IL-2) and interleukin-10 (IL-10) in serum with ELISA, the positive expressions of EGF, PDGF, bcl-2 and bax protein in the focal tissue with immunohistochemical method, and the positive expressions of bcl-2 mRNA and bax mRNA in the focal tissue with In situ hybridization.Result:1. The level of IL-2 in serum was lower in EM group than that in normal group, while the level of 11-10 was higher.2.The positive expressions of bcl-2 mRNA and protein in the focal tissue were higher in EM group than that in normal group, while the positive expressions of bas mRNA and protein were lower.3.The positive expressions of EOF and PDGF protein in the focal tissue were higher in EM group than that in normal group.Conclusion:1 .The imbalance of immune system may be related to the occurrence and development of EM.2.The abatement of programmed cell death in ectopic endometrium may play an important role in the development of EM.3.The growth of new vessels in ectopic endometrium could promote the implantation and growth of ectopic endometrium.4.YWN could regulate the imbalance of immune system, induce programmed cell death in ectopic endometrium, and inhibit the growth of new vessels in ectopic endometrium.更多还原