【作者】 周建国；

【导师】 赵德明；

【作者基本信息】 中国农业大学 ， 预防兽医， 2013， 博士

【摘要】 本研究采用猪口蹄疫(FMD)灭活疫苗和合成肽疫苗、猪瘟(CSF)脾淋活疫苗和高致病性猪蓝耳病(HPPRRS)舌疫苗同步分点(三点、两点)免疫技术,对被免疫猪只分别进行了小组试验、攻毒保护、免疫持续期观察、扩大应用和临床免疫等试验研究,并用ELISA方法定期对各阶段血清抗体进行检测,旨在探索一种适合基层应用的FMD, CSF. HPPRRS三种疫苗同步分点注射免疫技术,提高免疫密度和免疫效果,降低基层防疫人员劳动强度,提高工作效率,破解基层强制免疫工作难题。本研究得出下列结论。1.建立了口蹄疫、猪瘟和高致病性猪蓝耳病三种疫苗同步分点(三点、两点)免疫技术方法并证明了其临床应用的可行性与科学性。2.口蹄疫、猪瘟和高致病性猪蓝耳病三种疫苗同步分点(三点、两点)免疫证明了三种疫苗同步分点免疫相互间未出现免疫干扰现象。同时,对应用三种疫苗同步两点免疫技术中各病种免疫效果的同步评估需在免疫后35-40天期间进行监测为佳。种公猪采用三种疫苗同步两点免疫技术免疫,免疫后需停止供精7-10天采集一次精液无害化处理后可正常供精。3.应用口蹄疫三种疫苗同步两点攻毒保护率猪瘟、口蹄疫合成肽疫苗、口蹄疫灭活疫苗、高致病性猪蓝耳病活疫苗与其单苗免疫攻毒保护率相一致。4.三种疫苗同步两点免疫技术列入云南省2013年重大动物疫病免疫方案,2013年上半年全省16个州市129个县市区共免疫生猪3206.31万头,抗体转阳率口蹄疫为77.85%,猪瘟为83.15%,高致病性猪蓝耳病为93.92%,均达到并高于国家要求。免疫应激反应102004头,反应率为0.32%,免疫应激反应死亡7926头,死亡率为0.25%o,较传统免疫方式下降45%左右。本研究证明了三种疫苗同步分点注射的可行性和科学性,并将三种疫苗同步两点免疫技术大面积应用于免疫工作,解决了困扰基层临床免疫的难点问题,为其临床应用提供了科学依据和数据支持,在生产实际的临床应用中具有重要意义。更多还原

【Abstract】 Inactivated vaccine and synthetic peptide vaccine of foot-and-mouth disease (FMD) vaccines, attenuated live vaccines for either classic swine fever (CSF) or porcine reproductive and respiratory syndrome (PRRS) were used in the studies described in this paper. Pigs were divided into groups and vaccinated by simultaneously inoculated the three vaccines at three or two sites. Vaccinated pigs were carried out the lasting immunity test, extension application, virus challenge test and clinical immunity test. ELISA tests were used to measure antibody responses in different times of post vaccination. The aim of the studies is to seek an alternative and easy applying method for grass root veterinaries to vaccinate pigs with FMD、CSF、PRRS vaccines, and to reduce labor cost, increase the percent of coverage and efficacy of the vaccines. The following conclusions have been achieved.1. This study established a new immunological technique for Foot and Mouth Disease (FMD), Classical swine fever and Porcine reproductive and respiratory syndrome (PRRS). That was, three vaccines simultaneously injected in different points (three points, two points). And the study also demonstrated the feasibility and scientificalness of this technique in clinical applications.2.The study showed that no immunity interference in this new technique. In addition, it is best to assess the immune effects of vaccines during the35-40days after immunization. Stud boars need to stop provide semen for7-10days after immunized by this technique, and the boars can normally provide sperm after harmlessly treated.3.In this technique, the respective protective efficacy rate of FMD vaccine, Classical swine fever and PRRS vaccine, were consistent with applying the single vaccine alone.4. The three vaccines two sites simultaneous vaccination technique had been used to the important animal disease vaccination programs in Yunnan province in2013. In the first half of2013,32,063,100pigs were vaccinated in129counties of16cities or prefectures in the province. The antibody conversion rates for all vaccines reached or exceed the national standard (FMD77.85%, swine fever83.15%, PRRS93.92%). The hypersensitive responsive numbers were102004(0.32%) pigs. The hypersensitive responsive death numbers were7926(0.25%o) pigs, reduced about45%compared with the traditional vaccination procedures.The studies testified that the three vaccines three sites or three vaccines two sites simultaneous vaccination technique are reliable for FMD, CSF and HPPRRS vaccination. The techniques have been widely applied in the rural area and showed more efficiency to solve the problem of vaccination difficulty, and also imposed great significance in the actual application.更多还原