【作者】 芦琳琳；

【导师】 张士璀；

【作者基本信息】 中国海洋大学 ， 海洋生物学， 2013， 博士

【摘要】 众所周知，机体的免疫应答能力随着衰老而逐渐下降。红景天苷（Salidroside,SDS），是一种由中国传统中药红景天（Rhodiola L.）植物根茎中提取的抗氧化成分，一直以来以抗衰老，促进健康等功能而著称。然而，这种功能的内在机制却还不太明了。本研究明确证实（1）SDS口服吸收可使老龄Wistar雄性大鼠脾内CD3+T细胞和CD4+辅助性T细胞显著增加；（2）SDS能显著增强老龄大鼠体内迟发型超敏反应（DTH反应），该反应是由T细胞介导的免疫反应；（3）SDS口服吸收能在不影响体内免疫平衡的基础上，显著性增加抗KLH特异性的IgG，IgG1和IgG2α的数量。以上实验结果证明SDS具有对抗免疫衰老的作用，进而具有延年益寿的功效。因此，SDS可以被用来帮助老年个体提高对疫苗的反应，促进老年个体细胞和体液介导的免疫应答。谷氨酰半胱氨酸连接酶（Glutamate cysteine ligase, GCL）是谷胱甘肽（Glutathione, GSH）生物合成过程中的限速酶。许多数据显示衰老过程可能与机体内GCL水平的下降有关。本研究结果表明在老龄大鼠肝脏，心脏及脑组织中，GCL催化亚基（GCLc）和调节亚基（GCLm）基因的表达均显著下降，GCL酶活也显著降低。然而，通过口服红景天苷，则能够逆转上述状况，使老龄大鼠肝脏、心脏、脑中GCLc和GCLm基因的表达显著增加，GCL的活性显著增强，GCLC与GCLM亚基的蛋白含量也明显升高。除此之外，通过该实验结果，认为利用红景天苷进行营养干预，可有助于老年个体提高其抗氧化能力。综上，本实验结果表明在老龄大鼠中，红景天苷通过促进免疫应答，对抗免疫衰老，提高抗氧化能力，从而发挥其抗衰老作用。更多还原

【Abstract】 It is well known that immune response decreases with aging. Salidroside (SDS),an antioxidant component isolated from the traditional Chinese medicine roserootRhodiola rosea, has been demonstrated to possess potent anti-aging andhealth-promoting activities. However, the mechanism underlying these activities ispoorly understood. In this study, we clearly demonstrated that (1) dietary intake ofSDS induced a considerable increase in total T cells (CD3+) and T helper cells (CD4+)in aged Wistar male rats;(2) SDS supplementation significantly increased the DTHresponse, a T cell-mediated immune response, in aged rats; and (3) SDSsupplementation remarkably promoted the production of total anti-KLH IgG,anti-KLH IgG1and anti-KLH IgG2in aged rats without disturbing immunehomeostasis. These indicate that SDS is able to counteract immunosenescence,thereby resulting in rejuvenation. Pratically, SDS may be used to help the elderly togenerate an improved response to vaccine with stronger humoral and cell-mediatedimmune responses.Glutamate cysteine ligase (GCL) is the rate-limiting enzyme in GSHbiosynthesis. Several lines indicate that aging process may be related to reduction inGCL in organisms. Here we demonstrated that expression of GCL catalytic subunit(GCLc) and modulatory subunit (GCLm) decreased significantly in the liver, heartand brain from aged rats (22.5-month-old), and GCL activity also declinedsignificantly in all the tissues from aged rats. Notably, the decreases in GCLc andGCLm expression, GCL activity and GCLc and GCLm contents in the livers, heartsand brains from aged rats were resersed by oral administration of salodroside, a strongantioxidant with rejuvenating activity. These data indicate that salidroside is capableof enhancing GCL activity in vivo in aged rats. It also suggests that late onsetnutritional interventions with salidroside may help elder subjects to generate an improved redox state.更多还原