口服槲皮素对兔耳增生性瘢痕的抑制作用

【作者】 黄久佐；

【导师】 陈宗基； 王晓军；

【作者基本信息】 北京协和医学院 ， 临床医学， 2009， 博士

【摘要】 研究背景：体外实验提示,槲皮素具有抑制瘢痕增生的作用。但至今尚无口服槲皮素抑制瘢痕的体内实验。研究目的：探索口服槲皮素对兔耳增生性瘢痕的抑制作用。研究方法：在9只新西兰白兔的耳朵上建立标准创面(每耳4个,共72个)。3只白兔每天用100mg槲皮素灌胃,3只白兔每天用36mg积雪苷灌胃(阳性对照),另3只白兔不做灌胃处理(阴性对照)。4周后收集标本,从瘢痕横截面最高点制作切片,用瘢痕面积和未受创伤的基线面积之比作为瘢痕增生指数。结果：对照组的瘢痕增生指数为1.67±0.02,积雪苷组为1.55±0.03,积雪苷组的瘢痕增生程度比对照组减少17.9%,有统计学显著性差异(p=0.003)；槲皮素组的瘢痕增生指数为1.59±0.03,瘢痕增生程度比对照组减少11.9%,但无统计学显著性差异(p=0.053)。结论：口服积雪苷可显著抑制兔耳模型瘢痕增生,口服槲皮素对增生性瘢痕的抑制作用尚待进一步实验证实。更多还原

【Abstract】 Background:In vitro observations suggested that quercetin had a potential for the treatment of excessive scars. There has been no study about the effect of oral quercetin on hypertrophic scarring.Objective:to examine the effect of oral quercetin on hypertrophic scarring in the rabbit ear model.Methods:Standardized wounds, four per ear, were created on the ears of nine New Zealand White rabbits, for a total of72scars. Three of the rabbits received daily ingestion of quercetin or asiaticoside, respectively. And the other three received no intervention. After4weeks, the resulting ear scars were harvested. Histological slides were prepared from the thickest cross-sections of the scars, and hypertrophic index was calculated by comparing the area of the scar to the baseline value of unwounded skin.Results:Among the rabbits treated with asiaticoside, the mean hypertrophic index was1.55±0.03, compared with1.67±0.02in the control group (p=0.003), representing a17.9%reduction in hypertrophic area. Among the rabbits treated with quercetin, the mean hypertrophic index was1.59±0.03, representing a11.9%reduction in hypertrophic area, but there’s no significant reduction in scar hypertrophy compared with the control group (p=0.053).Conclusions:Orally administered asiaticoside significantly reduces the severity of hypertrophic scarring in the rabbit ear model. The inhibitory effect of oral quercetin on hypertrophic scarring still needs further investigation.更多还原