【作者】 张彦锋；

【导师】 李殿荣；

【作者基本信息】 西北农林科技大学 ， 生物化学与分子生物学， 2010， 博士

【摘要】 本研究以芸薹属不结球白菜雄蕊心皮化雄性不育系HGMS和甘蓝型油菜无花瓣雄蕊心皮化雄性不育系AMS为试材,进行了遗传和分子生物学研究。同时,对AP3基因决定花瓣形成的关键位点进行了转基因研究,取得了以下主要研究结果:1不结球白菜雄蕊心皮化不育系HGMS雄蕊心皮化性状受2对隐性基因控制;甘蓝型油菜无花瓣雄蕊心皮化不育系AMS雄蕊心皮化性状受4对隐性基因控制;?2 2类AP3基因(BAP3和BAP3_-24)共同调控芸苔属植物花瓣和雄蕊的发育。BAP3和BAP3_-24基因高度同源,仅有一处24bp插入/缺失明显差异,但两者的功能却显著不同,BAP3具有形成花瓣、雄蕊的双重功能,而BAP3_-24仅具有形成雄蕊的功能和促进萼片化花瓣发育的剂量效应。3不结球白菜含有BAP3和BAP3‐24两个基因,这两个基因功能缺失是引起HGMSa雄蕊心皮化突变的根本原因。4甘蓝型油菜含有2个BAP3和2个BAP3_-24。2个BAP3基因功能缺失,是引起甘蓝蓝型无花瓣油菜Apt花瓣缺失的主要原因。4个基因功能全部缺失,是引起AMSa雄蕊心皮化突变的根本原因。5转基因初步表明:24 bp特异序列是AP3基因决定花瓣形成的关键位点。更多还原

【Abstract】 Using stamen carpelloid male sterile lines HGMS of Brassica chinensis and AMS of apetalous Brassica napus as materials, they were studied from genetics and molecular biology. Subsequently, transgenic researches were been done to discove the potential key locus of AP3 genes, which was probably related to petal formation. The main results as follows:1 Stamen carpelloid phenotype of HGMSa mutant wss controlled by two pairs of recessive genes, while stamen carpelloid phenotype of AMSa mutant was controlled by four pairs of recessive genes;2 There were two kinds of different AP3 genes, BAP3 and BAP3_-24, specifying petal and stamen development in Brassica. BAP3 shared higher homology with BAP3_-24 except one notable difference of 24 bp insertion at their C-terminus, but had a remarkable difference in their functions. BAP3 specified petal and stamen development, while BAP3_-24 only specified stamen development and has little effect on petal formation, but had dose effect in sepaloid petals development;3 Brassica chinensis contained one BAP3 gene and one BAP3_-24 gene. Loss-of-functions of the two AP3 genes thoroughly were the reason to stamen carpelloid mutant HGMSa;4 Brassica napus contained two BAP3 genes and two BAP3_-24 genes. Loss-of-functions of the two BAP3 genes were the key reason to causing Apt petal loss deficient. While loss-of-functions of all the AP3 genes were the primary reason to stamen carpelloid mutant AMSa of apetalous B.napus;5 Transgenic results inferred preliminary that the special 24bp sequence between BAP3 and BAP3_-24 was the key locus of AP3 gene to specify petal development.更多还原