【作者】 韩秀丽；

【导师】 汪冰；

【作者基本信息】 山东中医药大学 ， 中医五官科学， 2011， 博士

【摘要】 目的：研究清瘤亮喉方对喉咽癌移植瘤生物学行为的影响,并探讨其治疗或干预喉咽癌的作用机制。方法：1.建立梨状窝兔VX2瘤模型兔：VX2瘤细胞株荷瘤新西兰大白兔传代,以传代兔肿瘤组织块,制成肿瘤组织液,直达喉镜下接种于兔梨状窝。2.研究清瘤亮喉方对喉咽癌移植瘤生物学行为的影响：新西兰大白兔46只接种肿瘤后,随机分为空白组、对照组(氟尿嘧啶组)、治疗组(清瘤亮喉方组),除7只种植兔意外死亡外,余39只进入研究。随后观察其生存情况、影像学(CT)、解剖学、病理学(HE染色)及免疫组化(细胞周期调控因子cyclinB、cyclinD、CDK4及P27)研究。结果：服用清瘤亮喉方组(治疗组)及氟尿嘧啶组(对照组)的梨状窝VX2瘤兔的生存质量及存活时间明显优于空白组(p<0.05)；治疗组与对照组的梨状窝VX2瘤兔的肿瘤生长情况在解剖及影像学上与空白组存在显著差异(p<0.05)；治疗组与对照组的梨状窝VX2瘤兔的肿瘤生长情况在病理学上与空白组存在显著差异(p<0.05)；治疗组与对照组、空白组的梨状窝VX2瘤兔的肿瘤生长情况在免疫组化研究上存在显著差异(p<0.05)。结论：1.清瘤亮喉方能有效改善梨状窝VX2瘤兔的生存质量,延长肿瘤兔的生命。2.清瘤亮喉方能干预喉咽癌移植瘤的生长,其机制可能是通过诱导肿瘤细胞凋亡。3.清瘤亮喉方对喉咽癌的治疗作用,体现了中药对机体免疫功能的调节。更多还原

【Abstract】 Objective:To Study of the effects on the biological behavior of the VX2 pyriformsinuscarcinoma(PSC) model with Qing Liu Ling Hou prescription; and to study the mechanism of the therapeutic effect with it.Methods:1. Establishment of Rabbit VX2 pyriformsinuscarcinoma model:To establish a rabbit VX2 hypolaryngocarcinom (HPC) model and observe it’s growth. After anesthesia,tumor tissues from the VX2 cancer New Zealand rabbit was implanted to the rabbits’groin to passage.Then tumor tissues from the passaged- rabbits,was made tumor tissue mass suspension, and the suspension was injected into rabbit’s pyriform sinus guided by rigid bronchoscope,2. The study of the efects on the biological behavior of the VX2 HPC model with Qing Liu Ling Hou prescription:46 rabbits of VX2 PSC model were divided into three groups in random:blank groups,cntrol groups of 5-F(FLUOROURACIL) and Chinese heb groups (Qing Liu Ling Hou prescription).7of the total 39 rabbits in the study died by accident. Therefore,the growth and metastatic character were observed by anatomy,CT and pathology. Immunohistochemistry staining by specific monoclonal anti-p27/Kipl protein,anti-cyclin D1 protein, anti-cyclin B protein and anti-CDK4 protein was performed using the S-P method..Results:1. The control groups and treatment groups in the living quality and duration were marked different than the blank groups.2. The control groups and treatment groups in anatomy and intervertebral were differently expressed as compared with those in blank groups. The control groups and treatment groups were significantly different with the blank groups in pathology. In immunology,the control groups were were significantly different with treatment groups, and the blank groups were significantly different with the control groups.Conclusion:1.To Qing Liu Ling Hou prescription; it can effectively improve the living quality, prolong the lifespan of the cancer rabbit.2. Qing Liu Ling Hou prescription can promote tumor-cells apoptosis in the VX2 PSC model by interventing expression of tumor-cells potentiality.3.The therapeutic principle of Qing Liu Ling Hou prescription may be based on promoting or inhibiting the expression of cellular potentiality,so it embodied the adjustion of the traditional chinese medicine on the body’s immune function.更多还原