【作者】 闫兆威；

【导师】 李平亚； Paul Groundwater；

【作者基本信息】 吉林大学 ， 生物化学与分子生物学， 2011， 博士

【摘要】 刺五加Acanthopanax senticosus (Rupr. et Maxim) Harms,又名五加参、刺拐棒,是一种非常珍贵的药用植物,在我国已有悠久的药用历史。《本草纲目》中称刺五加为“本经上品”,能够“补中益气,坚筋骨强意志,久服轻身耐老”,其根、茎、叶和果实均可供药用。近年来,国内外对刺五加的研究多集中在根、茎、叶部位,很少有文献报道对其果肉进行研究。因此本论文对刺五加果肉的化学成分及药理活性进行了系统研究。首次利用现代的分离纯化手段从刺五加果肉的水提物中分离得到45个化合物,依据其理化性质及波谱数据解析(1H NMR、13C NMR、DEPT、HMBC、HSQC、MS等),鉴定了40个化合物的结构,其中3个化合物为未见文献报道的新化合物,1个化合物为首次从自然界分得的新的天然产物,18个化合物为首次从该植物获得。首次采用气相色谱-质谱联用(GC-MS)技术对刺五加果肉中分得的油状物成分进行了分析,通过标准质谱图数据库检索,鉴定了其中13种成分。首次对刺五加果肉水提物进行了抗心律失常的药理作用研究。通过给Wistar大鼠舌下静脉注射氯化钡来复制心律失常实验动物模型,研究了刺五加果肉水提物对氯化钡致心律失常的影响。实验结果表明：刺五加果肉水提物能够明显缩短氯化钡诱发的大鼠室性心律失常持续时间,对心脏具有明显的保护作用,故本研究成果为今后开发刺五加果肉成为新的药用部位和新的抗心率失常药物提供了详实的科学依据。更多还原

【Abstract】 Acanthopanax senticosus (Rupr. et Maxim) Harms is a medicinal plant and belongs to the Araliaceae family, which has long been used as a folk medicine to treat many diseases in China. At present, most of the chemical and pharmacological studies are mainly focused on the roots, stems and leaves of Acanthopanax senticosus, and only a few reports relate to the pulp. In order to find more new active constituents from the pulp of A. senticosus, the chemical study on the water extract of the pulp of this plant was carried out. By various methods of isolation,45 compounds were isolated, and by the means of the physicochemical properties and spectroscopic methods(1HNMR,13C NMR、DEPT、HMB、HSQ、MS), 40 compounds were elucidated as:3,4-seco-lupane-20(29)-ene-3,28-dioic acid (1); (3S,4S, 5R)-3-octadecanoylamino-4-hydroxy-5-dodecane-2,3,4,5-tetrahydrofuran (2); (3S, 4S,5R)-3-[(2R)-2-hydroxyhexacosanoylamino]-4-hydroxy-5-[(4E)-dodecane-4-ene]-2,3,4,5-tetrahy drofuran (3); (-)-sesamin (4); (-)-schisandrin B (5); (-)-syringaresinol (6); (-)-3,4-divanillyltetrahydrofuran (7); (-)-savinin (8); Pluviatilol (9); Eleutheroside E (10); 6,7-dimethoxycoumarin (11); 7-hydroxy-6-methoxycoumarin (12); Hederasaponin B (13); 5a,8a-epidioxy-(22E,24R)-ergosta-6,22-dien-3β-ol (14);β-sitosterol (15); Stigmasterol (16); Stigmasterol palmitate (17); Daucosterol (18); Eleutheroside B (19); 2-furoic acid (20); Succinic acid (21); Catechol (22);D-sucrose (23); Uracil (24); Rutin (25); Phthalic acid bis-(2-ethyl-hexyl) ester (26); Glyceryl palmitate (27); Glyceryl monostearate (28): Pentadecanoic acid heptadecyl ester (29); Sorbitan monostearate (30); 12-hydroxy octadecanoic acid (31); 4-hydroxyoctadecanoic acid (32); 3-hydroxy hexadecanoic acid (33); 6-hentriacontanol (34); Undecanoic acid (35); 1,1,3,3-tetramethylcyclopentane (36): Pentadecane (37); Octadecane (38); Isoselachoceric acid (39):and 3,7-dimethyldecane (40), respectively. Among of them,compound 1,2.3 were identified as new compounds. compound 31 was identified as a new natural compounds, and compounds 5,7,9,11,12,14, 16,17,20,21,22,24,26,30,32,34,36,40 were obtained from the A. senticosus for the first time.At the same time, the liposoluble components from the pulp of A. senticosus were analyzed by GC-MS computer technology.63 peaks were obtained and 13 of them were identified by means of indexing the spectrum storeroom for the first time.The effects of water extract of pulp on experimental arrhythmias induced by barium chloride (BaCl2) were investigated in experimental Wistar mice. The results showed that water extract of the pulp of A. senticosus could remarkably shorten the duration time of arrhythmias induced by barium chloride, and prevent the development of heart disease. Thus, the research results could provide an exact scientific basis for R&D of the pulp of A. senticosus as new medicinal part and new anti-arrhythmias drugs in the future.更多还原